The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 4 to 8 have been flanked by loxP sites (size of loxP-flanked region: approx. 3 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 4 to 8 should result in loss of function of the Gckr gene by deleting the region containing the amino acids 96-215 and by generating a frameshift from exon 3 to exon 9.
According to NCBI and Ensembl databases, the Fndc4 gene (NCBI ID: 64339, Ensembl: ENSMUSG00000038552) is located approx. 3 kb upstream of Gckr exon 4 in a head-to-head orientation.
The corresponding constitutive KO allele is available as line 9896.
Datamining and Design performed in 2008.
Gene Family: Isomerase
Genetic Background: C57BL/6
Gene Symbol: Gckr
Gene Accession Number: NM_144909.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: glucokinase regulatory protein