May be used to generate constitutive or conditional knockouts of the Dll1 gene by crossing to the cre line of choice.
Dll1 is a Notch ligand
Useful for studies of Notch signaling, development, immune system development and function as well as autoimmune disease studies
Origin: The Floxed Dll1 Mouse was developed by Katsuto Hozumi in the laboratory of Michael Owen at the Laboratory of Lymphocyte Molecular Biology, Cancer Research UK, and further developed and characterized by Rachael Brooker in the laboratory of Julian Lewis at the Vertebrate Development Laboratory, Cancer Research UK. The model was generated by targeting the Dll1 gene to generate a floxed allele in 129-derived ES cells. The targeting vector includes a loxP site inserted before exon 3 and a loxP-flanked resistance cassette after exon 4. Properly targeted ES cells were transfected with a Cre expression vector to excise the resistance cassette and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice. Taconic received embryos from CRT in 2013.
Source: Sponsor: Cancer Research Technology
Availability: This model is available for immediate cryorecovery.
Hozumi K, Negishi N, Suzuki D, Abe N, Sotomaru Y, Tamaoki N, Mailhos C, Ish-Horowicz D, Habu S, Owen MJ. (2004) Delta-like 1 is necessary for the generation of marginal zone B cells but not T cells in vivo. Nat Immunol. 5(6):638-44