The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
Fabp4 exon 1 contains the translation initiation codon.
Exons 2-3 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 2-3 should result in loss of function of the Fabp4 gene by deleting most of the functional domain.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2004.
Gene Family: Enzyme activator
Genetic Background: C57BL/6
Gene Symbol: Fabp4
Gene Accession Number: NM_024406
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: fatty acid binding protein 4, adipocyte