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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
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Fabp4 exon 1 contains the translation initiation codon.
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Exons 2-3 have been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exons 2-3 should result in loss of function of the Fabp4 gene by deleting most of the functional domain.
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The remaining recombination sites are located in intron regions of the genome.
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Datamining and Design performed in 2004.
Gene Family:
Enzyme activator Genetic Background:
C57BL/6 Gene Symbol:
Fabp4 Gene Accession Number:
NM_024406 Allele Type:
KO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
fatty acid binding protein 4, adipocyte
