The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 2 to 8 have been flanked by loxP sites (size of loxP-flanked region: 2.4 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 2 to 8 should result in loss of function of the Elovl1 gene by deleting the complete ORF
According to NCBI and Ensembl databases, the Cdc20 gene (Entrez ID: 107995; ENSMUSG0000006398) is located downstream of Elovl1 in a tail-to-tail orientation. The 3´ UTR of Elovl1 gene overlaps with the 3´ UTR of Cdc20 (40 bp). Therefore, the distal LoxP site has to be inserted within Cdc20 exon 10 and would interfere with normal expression and stability of Cdc20 mRNA. In order to restore Cdc20 exon 10, 241 bp encompassing the overlapping 3´ UTR region has been duplicated in the targeting vector.
Datamining and Design performed in 2009.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Elovl1
Gene Accession Number: NM_019422.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1