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The targeting vector has been generated using C57BL/6J DNA and transfected into the C57BL/6NTac ES cell line.
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Dgat1 exon 1 contains the translation initiation codon.
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Exons 3 to 17 have been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of the exons 3 to 17 should result in loss of function of the Dgat1 gene by deleting the MBOAT domain and by removing the 3 ´untranslated region.
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The remaining recombination sites will be located in non-conserved regions of the genome.
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Datamining and Design performed in 2010.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Symbol:
Dgat1 Gene Accession Number:
NM_010046 Allele Type:
KO Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
diacylglycerol O-acyltransferase 1s
