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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 3 to 5 have been flanked by loxP sites (size of loxP-flanked region: 1.6 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of the exons 3 to 5 should result in the loss of function of the Cyp11b2 gene by deleting part of the catalytic domain and by generating a frameshift from exon 2 to exon 6 (premature Stop codon in exon 6).
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According to NCBI and Ensembl databases, the Cyp11b1 gene (Entrez ID:110115 ; ENSMUSG00000075604) is located approx. 11 kb downstream of Cyp11b2 exon 5, in a tail-to-head orientation.
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The corresponding conditional KO allele is available as line 11298.
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Datamining and Design performed in 2010.
Gene Family:
Synthase Genetic Background:
C57BL/6 Gene Symbol:
Cyp11b2 Gene Accession Number:
NM_009991.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
cytochrome P450, family 11, subfamily b, polypeptide 2
