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CRE-Luc GPCR Reporter Mouse, Line 11
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Overview
Nomenclature: FVB/NTac-Tg(CRE/Tk-luc)11Hgp
- Expresses luciferase under the control of the cAMP response element (CRE)
- GPCR signaling through the cAMP pathway can be detected from the target. GPCR that is in a native cellular environment with a full complement of associated receptors and membrane constituents
- Luciferase is expressed primarily in the pancreas in Line 11
- Useful for screening in vivo, in vitro, and ex vivo using whole animals, tissues, and primary cells
- Learn more about the CRE-Luc GPCR Reporter Mouse Platform
Origin
The CRE-Luc GPCR Reporter Mouse, Line 11 was developed by Sanofi. The model was created by microinjecting a transgene containing a 6x CRE promoter, the HSV TK minimal promoter, modified firefly luciferase cDNA from pGL4 and a human growth hormone polyA tail. This transgene was microinjected into FVB/NTac zygotes. The resultant mice from founder line 11 were bred to FVB/NTac mice. Taconic received stock from Sanofi in 2012. The line was embryo transfer derived. In the production colony, the line was maintained by mating homozygous males and females.
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 12 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
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Genetics
Guides & Publications
Initial Publication:
- Pang Z, Wu N, Zhang X, Avallone R, Croci T, Dressler H, Palejwala V, Ferrara P, Tocci MJ, Polites HG. (2010) GPR40 is partially required for insulin secretion following activation of b3-adrenergic receptors. Mol Cell Endocrinol 325(1-2):18-25.
- Dressler H, Economides K, Favara S, Wu NN, Pang Z, and Polites HG. (2014) The CRE luc Bioluminescence Transgenic Mouse Model for Detecting Ligand Activation of GPCRs. Journal of Biomolecular Screening, 2014, 19: 232.
- Download materials presented at the Keystone Symposium on G Protein-Coupled Receptors: Molecular Mechanisms and Novel Functional Insights, Banff, Canada, February 20, 2012. Presentation by Dr. Greg Polites, Sanofi: "The CRE-Luc Reporter Mouse Model: A Transgenic Bioimaging Mouse Model that can Assay Ligand Activation of GPCRs", February 20, 2012. View the accompanying poster.
Applications & Therapeutic Areas
- Diabetes
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CRE-Luc GPCR Reporter Mouse, Line 11
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.
This model is sold under terms which grant perpetual use rights.
This model is sold under terms which grant perpetual use rights.
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