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Expresses luciferase under the control of the cAMP response element (CRE)
GPCR signaling through the cAMP pathway can be detected from the target. GPCR that is in a native cellular environment with a full complement of associated receptors and membrane constituents
Luciferase is expressed primarily in the pancreas in Line 11
Useful for screening in vivo, in vitro, and ex vivo using whole animals, tissues, and primary cells
Learn more about the CRE-Luc GPCR Reporter Mouse Platform
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background: FVB Background
Origin: The CRE-Luc GPCR Reporter Mouse, Line 11 was developed by Sanofi. The model was created by microinjecting a transgene containing a 6x CRE promoter, the HSV TK minimal promoter, modified firefly luciferase cDNA from pGL4 and a human growth hormone polyA tail. This transgene was microinjected into FVB/NTac zygotes. The resultant mice from founder line 11 were bred to FVB/NTac mice. Taconic received stock from Sanofi in 2012. The line was embryo transfer derived. In the production colony, the line was maintained by mating homozygous males and females.
- Pang Z, Wu N, Zhang X, Avallone R, Croci T, Dressler H, Palejwala V, Ferrara P, Tocci MJ, Polites HG. (2010) GPR40 is partially required for insulin secretion following activation of b3-adrenergic receptors. Mol Cell Endocrinol 325(1-2):18-25.
- Dressler H, Economides K, Favara S, Wu NN, Pang Z, and Polites HG. (2014) The CRE luc Bioluminescence Transgenic Mouse Model for Detecting Ligand Activation of GPCRs. Journal of Biomolecular Screening, 2014, 19: 232.
- Download materials presented at the Keystone Symposium on G Protein-Coupled Receptors: Molecular Mechanisms and Novel Functional Insights, Banff, Canada, February 20, 2012. Presentation by Dr. Greg Polites, Sanofi: "The CRE-Luc Reporter Mouse Model: A Transgenic Bioimaging Mouse Model that can Assay Ligand Activation of GPCRs", February 20, 2012. View the accompanying poster.