Car Knockout Mouse

Constitutive Knockout

Car Constitutive Knockout Mouse Model
EZcohort® Models
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  • Model #
  • Genotype
  • Nomenclature
  • 9103-F
    ko/ko
    C57BL/6-Nr1i3tm1.1Arte
  • 9103-M
    ko/ko
    C57BL/6-Nr1i3tm1.1Arte
  • This targeted mutation strain carries a deletion of the mouse Nr1i3 gene which encodes for the nuclear receptor CAR.
  • Useful in defining the effect of CAR on CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy.

Origin:

The Car Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing a CAR humanized mouse line (Model 9101) with a PhiC31 deleter mouse. The Humanized CAR Mouse model was created through a knock in of a human CAR construct containing the genomic sequence from the translational start site on exon 2 to exon 9 onto the ATG of murine Car in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The Car Knockout Mouse model was derived from the Humanized CAR Mouse line through a PhiC31-mediated deletion of the human CAR sequence. This deletes all exons coding for functional domains of CAR (exons 3-9). The absence of CAR protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.

Color:

Black

Species:

Mouse

Initial Publication:

Scheer N, Ross J, Rode A, Zevnik B, Niehaves S, Faust N, Wolf CR. (2008) A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response. J. Clin. Invest. 118(9): 3228-3239.


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This model is sold under terms which grant perpetual use rights.