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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 2 contains the translation initiation codon.
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Exons 2 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 2 to 6 should result in loss of function of the Agpat1 gene by deleting the translation initiation codon, the transmembrane domains and the catalytic domain.
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Agpat1 gene:
- The Rnf5 gene (Entrez ID: 54197; ENSMUSG00000015478) is located approx. 6,8 kb upstream of Agpat1 exon 2 in a head-to-head orientation.
- The Egfl8 gene (Entrez ID: 81701; ENSMUSG00000015467) is located approx. 1,4 kb downstream of Agpat1 exon 6 in a tail-to-tail orientation.
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Datamining and Design performed in 2009.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Symbol:
Agpat1 Gene Accession Number:
NM_001163379 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)
