The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 2 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 2 to 6 should result in loss of function of the Agpat1 gene by deleting the translation initiation codon, the transmembrane domains and the catalytic domain.
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Agpat1 gene:
- The Rnf5 gene (Entrez ID: 54197; ENSMUSG00000015478) is located approx. 6,8 kb upstream of Agpat1 exon 2 in a head-to-head orientation.
- The Egfl8 gene (Entrez ID: 81701; ENSMUSG00000015467) is located approx. 1,4 kb downstream of Agpat1 exon 6 in a tail-to-tail orientation.
Datamining and Design performed in 2009.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Agpat1
Gene Accession Number: NM_001163379
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)