The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exon 4 has been flanked by loxP sites (size of loxP-flanked region: 1.2 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 4 should result in loss of function of the Acsm4 gene by deleting part of the catalytic domain and by generating a frameshift from exon 3 to all downstream exons (premature Stop codon in exon 5).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, the Thumpd1 gene (Entrez ID: 233802, ENSMUSG00000030942) is located approx. 16 kb downstream of Acsm4 exon 4 in a tail-to-tail orientation.
Datamining and Design performed 2009.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acsm4
Gene Accession Number: NM_178414.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: acyl-CoA synthetase medium-chain family member 4