The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Acly exon 2 contains the translation initiation codon.
Exons 17-19 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 17-19 should result in loss of function of the Acly gene by deleting the ATP-citrate lyase/succinyl-CoA ligase domain.
The remaining recombination sites are located in intron regions of the genome.
The conditional KO allele is also available as line 7917.
Data mining and Design performed in 2004.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acly
Gene Accession Number: NM_134037
Allele Type: KO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: ATP citrate lyase