Knock-in Mice

Knock-In Mice Create knock-in mouse models to streamline drug development, model human metabolism of compounds, and explore pathogenic mechanisms.

The exclusive package of homologous recombination technologies gives Taconic Biosciences the ability to perform the largest genomic insertions in the industry, on the most diverse selection of genetic backgrounds.

  • Model human genetic mechanisms
  • Identify transcriptional regulation elements
  • Track gene expression
  • Add molecular genetics tools to your model
  • Trust the most rigorous quality control in the field.

Design your own conditional, constitutive, and humanized knock-in mice or consult Taconic's murine genetics experts. We'll take your custom-engineered mouse model from concept to implementation, with seamless integration into the ExpressMODEL™ rapid breeding program.

Constitutive Knock-in Mice

Express your gene of interest throughout the development and life of your model.

Constitutive Knock-In Allele

Constitutive Knock-In with Optional Conditional KO

Exercise finer control over the behavior of your knock-in mutation.

Humanized Conditional Allele

Humanized Knock-in Mice with Optional Conditional KO

Exchange murine genes with their human orthologs to create humanized mouse models of disease, immunology, and physiology.

Humanized Conditional Allele

Conditional Knock-in Mice

Control time- or tissue-specific expression of exogenous mechanisms.

Conditional Knock-In Allele

Knock-In Mouse Generation

Taconic's knock-in mouse generation protocols take your custom-engineered mouse models from design to cohort with a unique approach to agile project management.

The Taconic Process

  • Target vector construction
  • Vector sequenced and electroporated into C57BL/6NTac ES cells (or other validated ES cell line).
  • Validate clones via Southern Blot
  • Inject validated first generation clones into mouse blastocysts for transfer to pseudopregnant females.
  • Breed first generation clones to deleter mice and remove selection markers.
  • Second-generation knock-in mice are germline competent and ready for delivery.
Throughout Taconic's agile project management process, you'll get regular updates from a dedicated project manager. Detailed progress reports are available at the completion of each project milestone.

Cohort Production Packages

All knock-in mouse cohort production packages include:
  • Documentation and verification of PCR-based genotype protocols.
  • Coordinated transfer of donor males to Taconic's breeding facility in Germantown, NY.
  • Confirmation of genotype of donor males by PCR-based genotyping.
  • Rapid Expansion using C57BL/6NTac donor females.
  • Holding of excess donor males until offspring from rapid expansion are weaned.
  • Comprehensive health testing with breeding while waiting for results of IHMS™-52 health testing.
  • PCR based genotyping of derived offspring.

Breeding to Single Gene Homozygotes

Package includes:
  • Barrier breeding of derived offspring in a heterozygous x heterozygous mating format.
  • PCR based genotyping of offspring from heterozygous x heterozygous mating.
  • Initial cohort of animals ready for shipment from 5 weeks of age on.
  • Large Cohort 8-10/sex/genotype
  • Small Cohort 4-6/sex/genotype
  • Genotypes include homozygous, heterozygous, and wild type.
Notes:
  • Package ends when initial cohort is ready for shipping. Post Package breeding and fees will be based on your breeding plan.
  • Shipping charges from Taconic to customer are not included in package.
  • Package assumes receipt of 4 donor males from Taconic, additional services will be charged at catalog prices.
  • Pricing assumes genetic modification is not sex linked, Mendelian ratios will be obtained, 80% of female breeders will deliver live pups that survive until weaning, and an average litter size of 5 pups. Additional charges may apply if these conditions are not met.

Conditional Knockout with Cre of Interest

  • Barrier breeding of derived offspring heterozygous for the conditional allele with your Cre line of interest.
  • PCR-based genotyping of offspring from above mating.
  • Barrier breeding of double heterozygous mice to generate appropriate experimental and control groups.
  • PCR-based genotyping of offspring from the above mating.
  • Initial cohort of animals ready for shipment at the beginning at 5 weeks of age.
  • Large package 8-10/sex/genotype
  • Small package 4-6/sex/genotype
  • Genotypes include homozygous for Cre-derived null deletion and wild type.
Notes:
  • Package ends when initial cohort is ready for shipping. Post package breeding and fees will be based on your breeding plan.
  • Shipping charges from Taconic to customer are not included in package.
  • Package assumes receipt of 4 donor males from Taconic. Additional services will be charged at catalog prices.
  • Package assumes use of Taconic ubiquitous general Cre Deleter mice (12524-T) will be used for crossbreeding. Additional fees may apply if different Cre mouse is used.
  • Pricing assumes genetic modification is not sex linked, Mendelian ratios will be obtained, 80% of female breeders will deliver live pups that survive until weaning, and an average litter size of 5 pups. Additional charges may apply if these conditions are not met.
The CIEA NOG mouse Download the Taconic Biosciences' Poster:
At Taconic Biosciences, we use both an in vivo strategy utilizing one-cell embryos and a complementary in vitro strategy utilizing embryonic stem (ES) cells to generate both mouse and rat models with CRISPR/Cas9. We provide a broad data-set to illustrate our experiences using these two strategies within our production pipeline.
Genetically Engineered Model Publications Database Search Taconic's GEMs Design database by application or model type:

GEMs Design inquiry Talk to a Taconic Biosciences scientist about designing a custom model:

Knock-in Mouse Publications:
Edwards JP, Thornton AM, Shevach EM. (2014) Release of active TGF-β1 from the latent TGF-β1/GARP complex on T regulatory cells is mediated by integrin β8. J Immunol. 193(6):2843-9.
Hasegawa M, Kapelyukh Y, Tahara H, Seibler J, Rode A, Krueger S, Lee DN, Wolf CR, Scheer N. (2011) Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line. Mol Pharmacol. 80(3):518-28.
Jun LS, Showalter AD, Ali N, Dai F, Ma W, Coskun T, Ficorilli JV, Wheeler MB, Michael MD, Sloop KW. (2014) A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor. PLoS One. 9(4):e93746.
McMillan SJ, Richards HE, Crocker PR. (2014) Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model. Immunol Lett. 160(1):11-6.
Ross J, Plummer SM, Rode A, Scheer N, Bower CC, Vogel O, Henderson CJ, Wolf CR, Elcombe CR. (2010) Human constitutive androstane receptor (CAR) and pregnane X receptor (PXR) support the hypertrophic but not the hyperplastic response to the murine nongenotoxic hepatocarcinogens phenobarbital and chlordane in vivo. Toxicol Sci. 116(2):452-66.
Scheer N, Wolf CR. (2014) Genetically humanized mouse models of drug metabolizing enzymes and transporters and their applications. Xenobiotica. 44(2):96-108.
Scheer N, Snaith M, Wolf CR, Seibler J. (2013) Generation and utility of genetically humanized mouse models. Drug Discov Today. 18(23-24):1200-11.
Scheer N, Balimane P, Hayward MD, Buechel S, Kauselmann G, Wolf CR.(2012) Generation and characterization of a novel multidrug resistance protein 2 humanized mouse line. Drug Metab Dispos. 40(11):2212-8.
Scheer N, Kapelyukh Y, Chatham L, Rode A, Buechel S, Wolf CR. (2012) Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines. Mol Pharmacol. 82(6):1022-9.
Scheer N, Kapelyukh Y, McEwan J, Beuger V, Stanley LA, Rode A, Wolf CR. (2012) Modeling human cytochrome P450 2D6 metabolism and drug-drug interaction by a novel panel of knockout and humanized mouse lines. Mol Pharmacol. 81(1):63-72.
Scheer N, Ross J, Kapelyukh Y, Rode A, Wolf CR. (2010) In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice. Drug Metab Dispos. 38(7):1046-53.
Scheer N, Ross J, Rode A, Zevnik B, Niehaves S, Faust N, Wolf CR. (2008) A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response. J Clin Invest. 118(9):3228-39.
Xu D, Rowland SE, Clark P, Giroux A, Cĉté B, Guiral S, Salem M, Ducharme Y, Friesen RW, Méthot N, Mancini J, Audoly L, Riendeau D. (2008) MF63 [2-(6-chloro-1H-phenanthro[9,10-d]imidazol-2-yl)-isophthalonitrile], a selective microsomal prostaglandin E synthase-1 inhibitor, relieves pyresis and pain in preclinical models of inflammation. J Pharmacol Exp Ther. 326(3):754-63.
Zhang J, Clark K, Lawrence T, Peggie MW, Cohen P. (2014) An unexpected twist to the activation of IKKβ: TAK1 primes IKKβ for activation by autophosphorylation. Biochem J. 461(3):531-7.
Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G. (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 9(3):e90570.