Beyond CRISPR: Choosing The Right Gene Modification Technology For Mouse Model Generation

While CRISPR has come to the forefront of many recent research discussions, multiple methods for creating genetically-engineered mouse models exist, explains Taconic Biosciences's Dr. Kenn Albrecht in a recent article in Cell & Gene. The most common techniques available to create a desired mouse model include:

  • Gene Targeting via Homologous Recombination
  • Random and Targeted Transgenesis
  • Gene Modulation via RNA Interference
  • CRISPR/Cas9 system
Gene targeting by homologous recombination (HR) is the "gold standard" technique that has existed for years. While it is extremely reliable, this method takes the longest from start to finish of all the techniques mentioned above. Two popular techniques are random and targeted transgenesis due to their ease and speed for model creation. CRISPR/Cas9 has enabled the generation of more complicated, specific knockouts and is exceedingly used for these purposes. RNA interference is the only technique mentioned above that is most useful for gene knockdowns versus gene knockouts due to its transient nature.

Each method has its associated benefits and drawbacks, which is why working with an experienced model generation company to achieve research project goals is of vital importance:

"It's difficult to discuss the genetic modification of animal models without CRISPR entering the conversation. While technologies like CRISPR/Cas9 have extended the boundaries of what is feasible for model development -- increasing the speed and efficiency with which genetic modification can be accomplished, while reducing costs -- the excitement and promise associated with CRISPR can create the misperception that it is always the best tool in the toolbox. This misperception can cause confusion, concern, or frustration for researchers who need a genetic modification for which CRISPR may not be the best approach."
Read the complete article at: cellandgene.com