CRISPR Gene Editing

CRISPR gene editing speeds timelines, reduces costs, and improves efficiencies in custom model generation.

  • Dramatically reduce timelines compared to traditional gene targeting methods.
  • Efficiently retarget existing animal models and repositories.
  • Generate custom knockouts and point mutations at significant cost reduction.

CRISPR How It WorksTaconic Biosciences uses the latest CRISPR/Cas9 gene editing technology to generate genetically-engineered animal models with a 100% success rate. Taconic holds CRISPR licenses from both UC Berkeley and the Broad Institute.

Contact us to talk with a Taconic scientist about applying CRISPR technologies to your animal model design program.

Generating CRISPR Mice and Rats

Taconic offers a mature, successful program for the design and generation of CRISPR-edited animal models.

Your CRISPR founders, fully characterized via genotyping and sequencing data, can be seamlessly transitioned to Taconic's rapid colony expansion and colony management programs.

  • Selection of target site, assays, and oligonucleotides, as well as predicted off-target sites.
  • Preparation of sgRNA and Cas9 mRNA.
  • Injection of sgRNA and Cas9 mRNA into mouse C57BL/6NTac zygotes, to generate F0 mutated candidate animals.
  • Genotyping and target-site sequence analysis in mutated candidate F0 animals.
  • Optional steps:
    • Germline breeding of F0 founder animal (optional)
    • Sequence analysis of germline transmitted F1 animals (optional)
    • Off-target analysis in germline transmitted F1 animals (optional)
Related White PaperDownload the Taconic Biosciences' White Paper:
The CIEA NOG mouseDownload the Taconic Biosciences' Poster:
At Taconic Biosciences, we use both an in vivo strategy utilizing one-cell embryos and a complementary in vitro strategy utilizing embryonic stem (ES) cells to generate both mouse and rat models with CRISPR/Cas9. We provide a broad data-set to illustrate our experiences using these two strategies within our production pipeline.
Genetically Engineered Model Publications Database Search Taconic's GEMs Design database by application or model type:

GEMs Design inquiry Talk to a Taconic Biosciences scientist about designing a custom model: