Knockout Mice

Knockout Mouse Vector Construction Use custom-engineered knockout mouse models to accelerate your research with insights into critical genetic mechanisms.

  • Explore cellular processes
  • Gain insights into disease pathogenesis and prevention
  • Streamline compound evaluation in drug discovery

Taconic Biosciences has successfully implemented over 575 conditional knockout model design projects over the last ten years. Design your own conditional and constitutive knockout mice or search Taconic's knockout repository.

Constitutive Knockout Mice

Create a mouse model where your gene of interest has been permanently deactivated throughout development.

Complete in approximately thirty-eight weeks.

Constitutive Knock Out Allele

Constitutive KO with the Option for Conditional KO

Design efficiently by generating constitutive and conditional knockout mice from the same gene-targeting event.

Complete in approximately forty weeks.

Conditional Knockout Mice

Perform more targeted preclinical studies by through time- or tissue-specific deletion of your gene of interest.

Complete in approximately forty weeks. Conditional Knock Out Allele

Conditional KO with Cre-ER Gene Switch

Silence gene expression at any stage of development with Taconic's proprietary Cre-ER technology.

Complete in approximately forty weeks.

Knockout Mouse Generation

Taconic's knockout mouse generation protocols accelerate delivery of study-ready animal models, modified to your design.

Starting with a gene accession number, Taconic's design team works with you to develop appropriate strategies for generating your mouse model.

The Taconic Process

All stages of Taconic's knockout model generation process are supported by Taconic's globally-recognized scientific support staff and implemented as part of a comprehensive agile development framework.

  • Target vector construction
  • Vector sequenced and electroplated into C57BL/6NTac ES cells (or other validated ES cell line).
  • Validate clones via Southern Blot
  • Inject validated first generation clones into mouse blastocysts for transfer to pseudopregnant females.
  • Breed first generation clones to deleter mice and remove selection markers.
  • Second generation knockout mice are germline-competent and typically ready for delivery at five weeks of age.
Study-ready, validated cohorts of knockout mice are typically available within thirty-eight to forty weeks of project approval.

Cohort Production Packages

All Cohort Packages Include:
  • Documentation and verification of PCR-based genotype protocol.
  • Coordinated transfer of donor males to Taconic's breeding facility in Germantown, NY.
  • Confirmation of genotype of donor males by PCR-based genotyping (max 2 alleles).
  • Rapid Expansion using C57BL/6NTac donor females.
  • Holding of excess donor males until offspring from rapid expansion are weaned.
  • Comprehensive health testing with breeding while waiting for results of IHMS-52 health testing.
  • PCR based genotyping of derived offspring (max 2 alleles).

Breeding to Single Gene Homozygotes

Package includes:
  • Barrier breeding of derived offspring in a heterozygous x heterozygous mating format.
  • PCR based genotyping of offspring from heterozygous x heterozygous mating (max 2 alleles).
  • Initial cohort of animals ready for shipment from 5 weeks of age on.
  • Large Cohort 8-10/sex/genotype
  • Small Cohort 4-6/sex/genotype
  • Genotypes include homozygous, heterozygous, and wild type.
Notes:
  • Package ends when initial cohort is ready for shipping. Post Package breeding and fees will be based on your breeding plan.
  • Shipping charges from Taconic to customer are not included in package.
  • Package assumes receipt of four donor males from Taconic, additional services will be charged at catalog prices.
  • Pricing assumes genetic modification is not sex linked, Mendelian ratios will be obtained, 80% of female breeders will deliver live pups that survive until weaning, and an average litter size of five pups. Additional charges may apply if these conditions are not met.

Conditional with Your Cre of Interest

Package includes:
  • Barrier breeding of derived offspring heterozygous for the conditional allele with your Cre line of interest.
  • PCR-based genotyping of offspring from above mating.
  • Barrier breeding of double heterozygous mice to generate appropriate experimental and control groups.
  • PCR-based genotyping of offspring from the above mating.
  • Initial cohort of animals ready for shipment at the beginning at 5 weeks of age.
  • Large package 8-10/sex/genotype
  • Small package 4-6/sex/genotype
  • Genotypes include homozygous for Cre-derived null deletion and wild type.
Notes:
  • Package ends when initial cohort is ready for shipping. Post package breeding and fees will be based on your breeding plan.
  • Shipping charges from Taconic to customer are not included in package.
  • Package assumes receipt of 4 donor males from Taconic. Additional services will be charged at catalog prices.
  • Package assumes use of Taconic ubiquitous general Cre Deleter mice (12524-T) will be used for crossbreeding. Additional fees may apply if different Cre mouse is used.
  • Pricing assumes genetic modification is not sex linked, Mendelian ratios will be obtained, 80% of female breeders will deliver live pups that survive until weaning, and an average litter size of 5 pups. Additional charges may apply if these conditions are not met.
Search current knockout mouse publications for more information relevant to your area of research.

The CIEA NOG mouse Download the Taconic Biosciences' Poster:
At Taconic Biosciences, we use both an in vivo strategy utilizing one-cell embryos and a complementary in vitro strategy utilizing embryonic stem (ES) cells to generate both mouse and rat models with CRISPR/Cas9. We provide a broad data-set to illustrate our experiences using these two strategies within our production pipeline.
Genetically Engineered Model Publications Database Search Taconic's GEMs Design database by application or model type:

GEMs Design inquiry Talk to a Taconic Biosciences scientist about designing a custom model:

Knockout Mouse Model Publications:
Maddaluno L, Rudini N, Cuttano R, Bravi L, Giampietro C, Corada M, Ferrarini L, Orsenigo F, Papa E, Boulday G, Tournier-Lasserve E, Chapon F, Richichi C, Retta SF, Lampugnani MG, Dejana E. (2013) EndMT contributes to the onset and progression of cerebral cavernous malformations. Nature. 498(7455):492-6.
Hansbro PM, Hamilton MJ, Fricker M, Gellatly SL, Jarnicki AG, Zheng D, Frei SM, Wong GW, Hamadi S, Zhou S, Foster PS, Krilis SA, Stevens RL. (2014) Importance of mast cell Prss31/transmembrane tryptase/tryptase-γ in lung function and experimental chronic obstructive pulmonary disease and colitis. J Biol Chem. 289(26):18214-27.
Metodiev MD, Spåhr H, Loguercio Polosa P, Meharg C, Becker C, Altmueller J, Habermann B, Larsson NG, Ruzzenente B. (2014) NSUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitoribosomal assembly. PLoS Genet. 10(2):e1004110.
Smeets MF, DeLuca E, Wall M, Quach JM, Chalk AM, Deans AJ, Heierhorst J, Purton LE, Izon DJ, Walkley CR. (2014) The Rothmund-Thomson syndrome helicase RECQL4 is essential for hematopoiesis. J Clin Invest. 124(8):3551-65.
Weber B, Schuster S, Zysset D, Rihs S, Dickgreber N, Schürch C, Riether C, Siegrist M, Schneider C, Pawelski H, Gurzeler U, Ziltener P, Genitsch V, Tacchini-Cottier F, Ochsenbein A, Hofstetter W, Kopf M, Kaufmann T, Oxenius A, Reith W, Saurer L, Mueller C. (2014) TREM-1 deficiency can attenuate disease severity without affecting pathogen clearance. PLoS Pathog. 10(1):e1003900.
Guo T, Marmol P, Moliner A, Björnholm M, Zhang C, Shokat KM, Ibanez CF. (2014) Adipocyte ALK7 links nutrient overload to catecholamine resistance in obesity. Elife. 3:e03245.