hTH-GFP Rat (Autosomal Insertion)

hTH-GFP (Autosomal Insertion) Rat Model

NTac:SD Background

  • Model #
  • Genotype
  • Nomenclature
  • 12108-F
    tg/wt
    NTac:SD-Tg(TH-EGFP)7Xen
  • 12108-M
    tg/wt
    NTac:SD-Tg(TH-EGFP)7Xen
  • Carries an EGFP transgene driven by the human Tyrosine Hydroxylase (hTH) promoter.
  • The hTH-GFP transgene is NOT located on the X chromosome. This is a key difference from model 12141 (X-linked transgene). Additionally, model 12108 exhibits ectopic GFP expression in olfactory bulb whereas the GFP expression in model 12141 (the X-linked version) has more specificity for TH neurons.
  • High level EGFP expression observed in dopaminergic neurons in brain structures such as, substantia nigra, ventral tegmental area, striatum, olfactory bulb, and hypothalamus, with minimal ectopic expression elsewhere in the brain.
  • Dopamine neurons are susceptible to damage/loss in Parkinson's disease and therefore this rat can be used as a tool to study damage/loss of dopamine neurons, e.g., after MPTP or 6-OHDA treatment.
  • Useful for in vivo anatomical visualization and micro-dissection of rat midbrain structures and axonal projections. High EGFP expression permits fluorescence imaging of brain slices.
  • Useful for FACS purification and in vitro culture of dopamine neurons for studies of disease pathogenesis in culture. Since EGFP is more easily detected than TH immunostaining at early developmental stages, this rat may be particularly useful for early embryonic studies of the development of dopamine neurons.
  • Since EGFP is more easily detected than TH immunostaining at early developmental stages, this rat may be particularly useful for early embryonic studies of the development of dopamine neurons.
  • Homozygous rats were not identified from a carrier x carrier mating format suggesting the model is homozygous lethal. Further analysis will be carried out and our website will be updated once more information is available. Carrier x WT mating format is currently recommended for breeding.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin:

The hTH-GFP rat was developed in collaboration between The Michael J Fox Foundation, Thomas Jefferson University, and Taconic. DNA fragments including 10.794 kb of the distal hTH promoter and 1.168 kb of proximal hTH promoter were ligated and placed upstream of the GFP reporter gene to create the hTH-GFP construct. This model was created through pronuclear injection of the hTH-GFP construct into NTac:SD zygotes. hTH-GFP founder line 7 (model 12108) was bred to NTac:SD for initial characterization. The colony is maintained by mating a NTac:SD female with a hemizygous male to produce hemizygous pups.

Source:

Model Sponsor: The Michael J. Fox Foundation

Availability:

Available off-the shelf. Limited breeding pairs available.

Color:

Albino

Species:

Rat

Initial Publication:

Iacovitti L, Wei X, Cai J, Kostuk EW, Lin R, et al. (2014) The hTH-GFP Reporter Rat Model for the Study of Parkinson’s Disease. PLoS ONE 9(12): e113151. doi:10.1371/journal.pone.011315



The hTH-GFP Rat Model may be purchased by non-profit users under the following label license (no signatures required).

LABEL LICENSE AGREEMENT FOR hTH-GFP RAT

The hTH-GFP Rat Model (“Model”) is produced and distributed under rights to patents and/or intellectual property owned by or licensed to the Michael J. Fox Foundation (“MJFF”) and Taconic. Taconic has rights to distribute the Model on behalf of MJFF. MJFF maintains ownership of the Model and permits the sale of such model to each purchaser that agrees to the Terms and Conditions of the Sale and following terms of use:

  • Title to the Model, any progeny, and biological materials derived from the foregoing remains with MJFF.
  • The Model may be bred or crossbred by purchaser
  • Purchaser shall inform MJFF of any crossbred animal generated (“Modified Progeny”), shall at MJFF's election deposit breeding pairs of each Modified Progeny with Taconic or another designee of MJFF and shall permit Taconic or another designee of MJFF to distribute the Modified Progeny on behalf of MJFF to non-profit institutions or for-profit institutions without any compensation to purchaser on terms similar to the terms and conditions by which purchaser received the Model. Purchaser assumes all liability for any claim or demand that Modified Progeny created by purchaser infringes on the intellectual property rights of any third party.
  • The Model, progeny, or Modified Progeny may be used for research purposes only.
  • The Model, any progeny, Modified Progeny, or biological materials will not be used for commercial purposes.
  • The Model, any progeny, Modified Progeny, and biological materials derived from them will not be distributed to any third party.
  • Purchaser assumes all risk from using the Model, any progeny, Modified Progeny, and biological materials.
  • To the extent permissible by law, purchaser indemnifies, defends, and holds harmless MJFF from any claim or demand that purchaser's use or transfer of the Model, any progeny, Modified Progeny, or biological materials has caused harm, damage, or liability.