DIO—Diet Induced Obese B6 Mouse Model

Nomenclature: C57BL/6NTac

Taconic Biosciences maintains an inventory of C57BL/6NTac male mice on 60% kcal high fat irradiated diet. Taconic uses Research Diets industry standard high fat rodent diet, D12492. C57BL/6NTac males are put on special diet at 6 weeks of age and housed at reduced density. Control males are housed in the same location, also at reduced density, and are fed NIH-31M diet. Control males can also be generated using a low fat purified diet upon request.

DIO mice in transit for an extended period of time (more than 48 hours) can lose 20-25% of their body weight and reach a new weight set point, making them resistant to high fat diet when they arrive. Taconic will only ship mice that have previously responded to DIO, but due to this known phenotype we suggest ordering an additional 5-10% in order to make sure you have a sufficient number of animals for your study.

To help mitigate the impact of extended transit on DIO phenotype stability, Taconic maintains regionally available cohorts of DIO mice. By sourcing animals from facilities geographically closer to your site, we reduce shipping times and help preserve the metabolic phenotype critical to your study's success. 

Animal Diet: DIO-B6-M are fed the D12942 diet and B6-DIOCONTROL-M are fed the NIH #31M Rodent Diet. If a low-fat purified ingredient diet is preferred, recommended control diets include D12450J and D12450K from Research Diets, though other variations are possible. It is recommended to have the diet on hand prior to animal receipt, as animals will be shipped with only enough diet for time in transit. 

Customized Services: In addition to the off-the-shelf DIO B6 offering, Taconic offers some customizable options upon request, including custom diet administration (ex: compound dosing via diet), body weight monitoring, extended cohort aging, food intake measurement, and special water administration (ex: fructose in water).