2. How Do I Plan My First Germ-Free Mouse Study?
Frank Razzaboni, from Park Bioservices, discusses axenic mouse study design in video below, from planning a successful germ-free project to vendor selection for required resources and components.
3. What Types of Monitoring are Required to Maintain Germ-Free Isolators?
Dr. Paula Roesch reviews monitoring of newly set up isolators, supplies, and testing post-animal entry.
In addition, you can also review a summary of husbandry differences
based on health standard.
2015 Symposium: Advancing Microbiome Research, Microbiome & Mice
4. What is the best method for testing prior to adding animals to the isolators?
As part of our Germ-Free Pilot program, Taconic Biosciences often hosts investigators on-site at our facility to review procedures in maintaining axenic mice. As a best practice, try a pilot study to assess your germ-free technique prior to starting a large cohort.
5. How Do I Test Isolators for Leaks?
In this video, Park Bioservices' Frank Razzaboni, demonstrates three methods for testing isolators for leaks.
6. How Many Mice Can Be Shipped in a Germ-Free Shipper?
Taconic can fit up to three 11.5″ x 7.5 ″ x 5″ cages into each germ-free shipper. The number of animals per shipper will vary based on animal age, so review the packing densities
and other details of the germ-free shipper.
7. What's the Best Way to Transfer Axenic Mice Into Isolators?
Taconic offers an informational summary on removing animals from a germ-free shipper
, as well as an instructional video demonstrating how to enter supplies and animals into an isolator.
8. What Physiological Differences Will I See in Germ-Free Versus Conventional Mice?
Watch the presentation
on unique characteristics of germ-free mice by Dr. Tamara Goode, Director, Animal Models Core, University of Pennsylvania.
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