The volume of published studies employing axenic or germ-free mice increased dramatically over the past 15 years, in part due to growing interest in microbiome research. As germ-free mouse model studies gain relevance, so does the importance of establishing best practices while researchers start and maintain research colonies.
Today, we'll address eight of the most frequently-asked questions regarding axenic mouse colony best practices.
In addition, you can also review a summary of husbandry differences based on health standard.
Today, we'll address eight of the most frequently-asked questions regarding axenic mouse colony best practices.
1. Can Germ-Free Mice be Maintained in Individually Ventilated Cages?
It is generally accepted that germ-free mice can be maintained for short durations in individually ventilated cages, however isolators should be used for maintaining the germ-free health standard for longer studies or for breeding projects.2. How Do I Plan My First Germ-Free Mouse Study?
Frank Razzaboni, from Park Bioservices, discusses axenic mouse study design in video below, from planning a successful germ-free project to vendor selection for required resources and components.3. What Types of Monitoring are Required to Maintain Germ-Free Isolators?
Dr. Paula Roesch reviews monitoring of newly set up isolators, supplies, and testing post-animal entry.In addition, you can also review a summary of husbandry differences based on health standard.