NOD

NOD Inbred Mouse Model
  • Live
  • Mouse
  • Male & Female Available
  • Albino
  • Available for direct purchase by CRO
Model No.NomenclatureGenotype
NOD-FNOD/MrkTac
NOD-MNOD/MrkTac
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Overview

Nomenclature: NOD/MrkTac

  • Exhibits destructive autoimmune pancreatic insulitis as early as four weeks of age
  • Diabetes incidence varies in this model based on a number of factors, including microbiome. The most recent phenotype monitoring data is posted below under Phenotypic Data. Phenotype can vary based on husbandry conditions, and Taconic cannot guarantee disease onset or incidence.

Availability

This model is not available to deliver at greater than 9 weeks of age for welfare reasons.

Origin

The NOD inbred model was developed by intercrossing ICR mice. The mice were inbred twenty generations by Makino. Taconic received stock in 1985. The mice were derived by hysterectomy. The Taconic foundation colony was at F76 in 2005.

Genetics

Species:Mouse
Strain Type:Inbred
Coat Color:Albino
Genetic Background:NOD

Other Genetic Notes:

  • Strain Profile: Acy1s, Car2a, Ce2a, Es1b, Es3c, Es6a, Esda, Es11a, Fv1b, Ggca, Gpi1a Gus-sb, H6pd1b, Hbap, Hbbs, Idh1a, Ldh1a, Ldh2a, Ldr1a, Mod1b, Mpi1b, Mup1a, Pepcb, Pgdb, Pgm1a, Pgm2a, Pnpa, Sod1a, Trfb
  • Immunology: H2g7
  • Other: Ptprca

Guides & Publications

Applications & Therapeutic Areas

  • Diabetes
  • Immunology
  • Inflammation
  • Metabolic Disease
  • Microbiome

Transit, Housing & Welfare

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Packing Practices

Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns.  When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.

Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age."  Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.

Murine Biospecimen Collection

mouse

Surgical Models and Services

mouse

Data

Average litter size: 9

2022 Diabetes Incidence in NOD Production Colony (IBU34)

Percentage of diabetes incidence and age in weeks in NOD mouse production colony

Taconic assessed diabetes incidence via urine analysis. Fresh urine was collected and applied to a Diastix test strip. Test strip results of ≥250 mg/dl of glucose in urine were considered diabetic. Results below 250 mg/dl were considered non-diabetic.

Data on diabetes onset and incidence in the NOD production colony is based on a snapshot in time. Diabetes phenotype in NOD mice may be affected by microbiome and other factors. Taconic cannot guarantee phenotypic performance.

NOD Physiological Data Summary (July 2004; N=10/group):

ParameterUnitsNOD MalesNOD Females
Serum ChemistryAvg ± S.D.   
Calciummg/dL10.6 ± 0.611.1 ± 0.3
Phosphorousmg/dL11.8 ± 2.010.2 ± 3.6
Glucosemg/dL116 ± 1888 ± 17
Creatininemg/dL0.1 ± 0.00.1 ± 0.1
BUNmg/dL22 ± 217 ± 6
Total Bilirubinmg/dL0.2 ± 0.10.1 ± 0.0
ALKU/L180 ± 11184 ± 21
ALT U/LU/L67 ± 6344 ± 11
Total Proteing/dL6.1 ± 0.66.2 ± 0.1
Blood Counts
Erythron   
Red Blood CellsX10^6/uL8.54 ± 0.5758.955 ± 0.476
Nucleated RBCX10^6/uL0 ± 00 ± 0
Hemoglobing/dL15.2 ± 0.515.1 ± 0.7
Hematocrit%46.7 ± 3.546.2 ± 2.5
MCVfL55 ± 152 ± 0
MCHpG17.8 ± 1.016.9 ± 0.2
MCHC%32.7 ± 2.132.7 ± 0.4
Platelets   
PlateletsX10^3/uL1165 ± 3341127 ± 291
Leukogram   
White Blood Cellsx10^3/uL3.654 ± 0.8755.438 ± 1.234
Neutrophilx10^3/uL1.211 ± 0.3262.011 ± 0.872
Bandsx10^3/uL0 ± 00 ± 0
Lymphocytex10^3/uL2.400 ± 0.9993.380 ± 1.139
Monocytesx10^3/uL0.039 ± 0.0680.016 ± 0.030
Eosinophilx10^3/uL0.003 ± 0.0100.031 ± 0.063
Basophilsx10^3/uL0 ± 00 ± 0
Othersx10^3/uL0 ± 00 ± 0
Organ Weights   
Stomachg0.275 ± 0.0160.257 ± 0.025
Ileumg0.791 ± 0.0620.799 ± 0.098
Colong0.206 ± 0.0420.192 ± 0.050
Lungsg0.228 ± 0.0240.226 ± 0.025
Heartg0.174 ± 0.0320.136 ± 0.017
Liverg1.139 ± 0.0720.955 ± 0.076
Spleen/Pancreasg0.228 ± 0.0320.193 ± 0.027
Kidney (L)g0.204 ± 0.0170.137 ± 0.011
Kidney (R)g0.204 ± 0.0170.138 ± 0.013
Testes (L)g0.103 ± 0.008-
Testes (R)g0.189 ± 0.268-
Ovary (L)g-0.020 ± 0.008
Ovary (R)g-0.021 ± 0.012
Urinalysis   
Glucosemg/dLNegativeNegative
Blood-NegativeNegative
PH-6.85 ± 0.416.45 ± 0.16
Proteinmg/dLNegativeNegative
Specific Gravity-1.01 ± 0.011.02 ± 0.00

Phenotyping Procedures:


Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line NOD (NOD/MrkTac), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH31 diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

 

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions, and the results are presented in Table 2.

 

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood and slides were delivered to LabCorp (RTP, NC) for analysis; the results are presented in Table 3. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.

 

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. Tabulation of organ weights is presented in Table 4. The pathologist's summary and detailed histological descriptions follow.

 

Discussion

All animals were thrifty on arrival and appeared clinically normal. Locomotor behavior was normal and there were no visible lesions or discharges at the mucous membranes. All tissues appeared normal on gross necropsy evaluation and weights were within normal limits. There were no unusual findings upon collection of these tissues nor otherwise observed in the body cavities.

The overall profile of these mice is consistent with expectations of a general-purpose mouse. None of the parameters are indicative of impaired system function.

Pricing - USD

Opportunist Free (OF) Health Standard

NOD Female

NOD-F

Age in WeeksQuantity 1 - 999
3 to 4$119.00
5 to 7$150.00
8 to 9$181.00

NOD Male

NOD-M

Age in WeeksQuantity 1 - 999
3 to 4$103.00
5 to 7$126.00
8 to 9$149.00

Pricing - EUR

Opportunist Free (OF) Health Standard

NOD Female

NOD-F

Age in WeeksQuantity 1 - 999
3 to 4€120,00
5 to 7€152,00
8 to 9€181,00

NOD Male

NOD-M

Age in WeeksQuantity 1 - 999
3 to 4€87,00
5 to 7€126,00
8 to 9€149,00

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