Pla2g4 - Model 11266 - TG

  • Cryopreserved
  • Mouse
  • Not available for direct purchase by CRO
Model No.NomenclatureGenotype
11266C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl
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Overview

Nomenclature: C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl

  • The transgenic construct contains 5´ the 8.5 kb mouse CaM-kinase II alpha chain (mCamk2a) promoter fragment, an 850 bp DNA fragment containing the human b-globin intron 2, and 2247bp ORF of human PLA2G4A cDNA (GenBank Acc# NM_024420), followed by 221bp of SV40 poly A sequence at the 3´ end in a pGL3 vector backbone (Promega).
  • A consensus KOZAK sequence has been added upstream of the starter ATG.
  • The plasmid backbone was removed and the transgenic construct was microinjected in C57BL/6 mice by pronuclear injection.
  • Putative transgenic founders were identified by genotyping and bred for germline transmission with C57BL/6 mice.
  • Heterozygous transgenic mice were identified by genotyping.

    • Line 11266, C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl, has the highest expression level of the transgene in the brain.
    • Line 11269, C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl, has the medium expression level of the transgene in the brain.

  • Datamining and Design performed in 2009.

Availability

Cryopreserved at Taconic

Source: Xenogen

This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.

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Genetics

Species:Mouse
Strain Type:Inbred
Allele Type:Random Transgenic
Genetic Background:C57BL/6
Gene Family: Phospholipase
Gene Symbol: Pla2g4
Gene Accession Number:  NM_008869
Gene Name: phospholipase A2, group IVA (cytosolic, calcium-dependent)

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Transit, Housing & Welfare

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Pla2g4 - Model 11266 - TG

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

    ItemCommercialNonprofit
    Cryopreserved Model$32,500$5,500
      ItemCommercialNonprofit
      Cryopreserved Model€29.500€5.005

       

      Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.

      Fees for Taconic Transit Cages™ and freight are in addition to the price above.

       

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