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The targeting vector has been generated using 129Sv DNA and transfected into 129Sv ES cell line.
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Tyrobp exons 3 and 4 were removed by the neomycin resistance cassette, which is flanked by loxP sites.
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The neomycin resistance gene has been deleted by transient transfection of Cre in targeted ES cells.
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Chimeric mice were produced and germline transmission achieved.
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Deletion of exons 3 and 4 should result in loss of function of the Tyrobp gene by deleting the transmembrane region and part of the cytoplasmic region.
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The remaining recombination sites are located in intron regions of the genome.
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See A.B.H. Bakker et al, Immunity 13, 345-353, 2000, for further details.
Gene Family:
Kinase Genetic Background:
C57BL/6;129SvEv Gene Symbol:
Tyrobp Gene Accession Number:
NM_011662 Allele Type:
KO Source:
DNAX Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
TYRO protein tyrosine kinase binding protein
