LRRK2 G2019S Mouse

Targeted Replacement

LRRK2 G2019S Mouse

LRRK2 G2019S Mouse Background

  • Model #
  • Genotype
  • Nomenclature
  • 13940-F
    ki/ki
    C57BL/6-Lrrk2tm4.1Arte
  • 13940-M
    ki/ki
    C57BL/6-Lrrk2tm4.1Arte
Model 13940 is a constitutive Knock-In (KI) of the mouse LRRK2 gene. The human G2019S point mutation has been introduced into exon 41 of the mouse LRRK2 gene.

  • The G2019S alpha-mutation is linked to the development of Parkinson's disease, both sporadic and familial1.
  • In patients, the G2019S LRRK2 mutation is an autosomal dominant mutation that leads to pathology similar to what is observed with idiopathic Parkinson's disease.
  • At 18 months of age, LRRK2 G2019S mice show an increased locomotor response after amphetamine challenge.
  • This knock-in mutation does not appear to affect basal motor function or more complex behavior in C57BL6 mice.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Availability: Ordering Note: Pricing to 8 weeks only. Requests for animals greater than 8 weeks of age is via Inquiry only.

Ordering Note for Controls: Model 13940 WT littermate controls are not available for this model due to the homozygous mating format. A recommended WT control option is to utilize C57BL/6NTac commercial animals sourced from our Cambridge City, Indiana production site (the same location where model 13940 is bred). Model 13940 has been generated and maintained on the C57BL/6NTac genetic background.
What our customers say:

“Robust, well-validated pre-clinical models of Parkinson's disease are a necessary tool for understanding the biology and possible treatment of this complex disease. With dedicated project management and technical support, Taconic has been a reliable partner in The Michael J. Fox Foundation's efforts to provide the research community with important, field-enabling pre-clinical models. We look forward to continuing to partner with Taconic for custom model generation projects.”
The Michael J. Fox Foundation

Genetic Background:

C57BL/6NTac Background

Origin:

To generate the constitutive KI Lrrk2tm4.2Arte mice, targeting vectors having proximal loxP and frt-flanked PGK–neomycin cassettes were inserted into intron 41; a distal loxP site was inserted into intron 42 of the endogenous mouse LRRK2 gene. The vectors were introduced into C57BL/6NTac-derived ES cells. Successfully targeted ES cells were injected into blastocysts, and then chimeric mice were bred with a Flp deleter strain to remove the selection neo cassette. Mice were then bred with C57BL/6NTac mice to remove the Flp transgene. The colony is maintained by mating a homozygous males and females. Taconic received stock from Artemis in 2007 and introduced it into barrier production after the mice went through embryo transfer derivation.

Source:

Model Sponsor: The Michael J. Fox Foundation

Color:

Black

Species:

Mouse

Initial Publication:

Matikainen-Ankney BA, et al. 2016. Altered Development of Synapse Structure and Function in Striatum Caused by Parkinson's Disease-Linked LRRK2–G2019S Mutation. J Neurosci. Jul 6; 36(27): 7128–7141. PMID: 27383589.


Label License Agreement and Conditions of Use for LRRK2 G2019S Mouse Model #13940

The LRRK2 G2019S Mouse Model ("Model") is produced and distributed under rights to patents and/or intellectual property owned by or licensed to the Michael J. Fox Foundation ("MJFF") and Taconic. Taconic has rights to distribute the Model on behalf of MJFF. MJFF maintains ownership of the Model and permits the sale of such Model to each purchaser that agrees to the Terms and Conditions of the Sale and following terms of use:

  • Title to the Model, any progeny and biological materials derived from the foregoing remains with MJFF.
  • Purchasers may breed the Model for their internal research purposes only, such as to obtain research cohorts or to obtain embryos or fetuses required for research purposes (e.g., for obtaining embryonic neurons).
  • Purchasers may not (i) cross-breed the Model except to produce wild type littermate controls by cross-breeding to Taconic's Model C57BL/6NTac, (ii) genetically alter the Model or Biological Materials, and (iii) establish or maintain a culture of any Biological Materials (e.g., cell lines containing luciferase), with the exception of primary cells (e.g., neurons).
  • The Model, progeny, or Modified Progeny may be used by non-profit institutions or for-profit institutions for research purposes only.
  • The Model, any progeny, Modified Progeny or biological materials will not be used for commercial purposes.
  • The Model, any progeny, Modified Progeny and biological materials derived from them will not be distributed to any third party.
  • Purchaser assumes all risk from using the Model, any progeny, Modified Progeny and biological materials.
  • To the extent permissible by law, purchaser indemnifies, defends, and holds harmless MJFF from any claim or demand that purchaser's use or transfer of the Model, any progeny, Modified Progeny or biological materials has caused harm, damage or liability.
Conditions of Use - Printable Version