IL-1β Knockout Mouse

Constitutive Knockout

IL-1β Knockout Mouse - Constitutive Knockout
This model is available for immediate cryorecovery.

  • Model #
  • Genotype
  • Nomenclature
  • 17188
    B6;129S7-Il1btm1Lvp
  • Carries a deletion of the interleukin 1β gene
  • Homozygous mice show an impaired acute phase inflammatory response and a reduced fever response to local inflammatory agents
  • Useful to examine the role of interleukin in inflammatory responses and for studies on arthritis

Allele Type:

KO

Origin:

The IL-1β knockout was developed by Zheng et al. in the laboratory of Lex Van der Ploeg at Merck Research Laboratories. AB 2.1 (129S7/SvEvBrd) embryonic stem cells, gene targeted by positive-negative selection, were injected into C57BL/6 blastocysts. The resultant chimeras were backcrossed for three generations to the MHC congenic B10.RIII-H2r to transfer the H2r haplotype to the targeted mutant. The IL-1ß heterozygotes were intercrossed to achieve homozygosity. This model was distributed as Taconic line 612, which is no longer available. The line was subsequently backcrossed by a third party to C57BL/6J and the H2r haplotype was removed. This current version of the model was imported to Taconic in 2018 and is congenic on C57BL/6J (NE7) with some residual genomic DNA from the 129 ES cells according to SNP testing.

Color:

Black

Species:

Mouse

Initial Publication:

Zheng, H.; Fletcher, D.; Kozak, W.; Jiang, M.; Hofmann, K. J.; Corn, C. A.; Soszynski, D.; Grabiec, C.; Trumbauer, M. E.; Shaw, A.; Kostura, M. J.; Stevens, K.; Rosen, H.; North, R. J.; Chen, H. Y.; Tocci, M. J.; Kluger, M. J.; Ploeg, L. H. V. D. Immunity 1995, 3 (1), 9–19.

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
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