- A new and improved B6 albino strain based on the C57BL/6NTac substrain.
- Contains two mutations, a mutation in the Tyr gene to introduce albinism, and a reversion of the non-agouti (a) locus to the agouti locus (A)
- Using the Taconic B6 Albino as a blastocyst host and mating the subsequent chimeras to a standard B6 mouse permits detection of ES cell germline transmission by coat color and ensures that the G1 animal is identical to the B6 genome at all coat color loci
- The Taconic B6 albino is an ideal host for maintaining substrain speciﬁcity ES cells derived from the C57BL/6NTac substrain
- Can also be used as a genetic background for models in which a B6 background is needed with an albino coat, for example in imaging applications
- Available only from Taconic!
- Please note that chimeras generated using B6-derived ES cells and blastocysts from the B6 Albino A++ will be tri-colored. The agouti signalling peptide (produced in blastocyst-derived cells, which appear albino in the chimera) can produce a paracrine signalling effect in some cells derived from the ES cells. ES cell-derived hair patches can thus be either black or agouti. Both black and agouti patches should be counted for determination of chimerism percentages.
Genetic Background: C57BL/6NTac Background
Origin: The B6 Albino A++ model was developed by Taconic. In C57BL/6 mice, intron 1 of the agouti locus contains a retrotransposon which is responsible for the non-agouti phenotype. In the Taconic B6, the region containing this retrotransposon was replaced with a FRT-flanked neomycin-puromycin resistance cassette in C57BL/6NTac-derived ES cells via gene targeting. The constitutive knock out allele was then generated via Flp-mediated removal of the selection cassette. The Tyr mutation is a constitutive knock in of a point mutation in which a G nucleotide located at position 369 in exon 1 has been replaced with C nucleotide, resulting in an amino acid substitution at position 103 (Cys>Ser). The F3 site flanked selection marker was removed by via Flp recombinase. Mice containing the agouti and tyrosinase mutations were intercrossed, and then the line was intracrossed to homozygosity.
Genetics: Use of the Taconic B6 Albino A++ in the following manner permits detection of ES cell germline transmission in a single step by coat color, with NO genotyping required!
Additionally, the germline progeny are identical to the C57BL/6 genome at all coat color loci!
Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G. (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 2014 Mar 5;9(3):e90570