B6 Albino A++

Targeted Replacement

B6 Albino A++ Targeted Replacement Mouse Model
EZcohort™ Models
The easiest way to acquire and breed cryopreserved GEMs
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C57BL/6NTac Background

  • Model #
  • Genotype
  • Nomenclature
  • 11227-F
    ko/ko;ki/ki
    C57BL/6NTac-Atm1.1Arte Tyrtm1Arte
  • 11227-M
    ko/ko;ki/ki
    C57BL/6NTac-Atm1.1Arte Tyrtm1Arte
  • A new and improved B6 albino strain based on the C57BL/6NTac substrain.
  • Contains two mutations, a mutation in the Tyr gene to introduce albinism, and a reversion of the non-agouti (a) locus to the agouti locus (A)
  • Using the Taconic B6 Albino as a blastocyst host and mating the subsequent chimeras to a standard B6 mouse permits detection of ES cell germline transmission by coat color and ensures that the G1 animal is identical to the B6 genome at all coat color loci
  • The Taconic B6 albino is an ideal host for maintaining substrain specificity ES cells derived from the C57BL/6NTac substrain
  • Can also be used as a genetic background for models in which a B6 background is needed with an albino coat, for example in imaging applications
  • Available only from Taconic!
  • Please note that chimeras generated using B6-derived ES cells and blastocysts from the B6 Albino A++ will be tri-colored. The agouti signalling peptide (produced in blastocyst-derived cells, which appear albino in the chimera) can produce a paracrine signalling effect in some cells derived from the ES cells. ES cell-derived hair patches can thus be either black or agouti. Both black and agouti patches should be counted for determination of chimerism percentages.

Genetic Background:

C57BL/6NTac Background

Origin:

The B6 Albino A++ model was developed by Taconic. In C57BL/6 mice, intron 1 of the agouti locus contains a retrotransposon which is responsible for the non-agouti phenotype. In the Taconic B6, the region containing this retrotransposon was replaced with a FRT-flanked neomycin-puromycin resistance cassette in C57BL/6NTac-derived ES cells via gene targeting. The constitutive knock out allele was then generated via Flp-mediated removal of the selection cassette. The Tyr mutation is a constitutive knock in of a point mutation in which a G nucleotide located at position 369 in exon 1 has been replaced with C nucleotide, resulting in an amino acid substitution at position 103 (Cys>Ser). The F3 site flanked selection marker was removed by via Flp recombinase. Mice containing the agouti and tyrosinase mutations were intercrossed, and then the line was intracrossed to homozygosity.


Genetics:

Use of the Taconic B6 Albino A++ in the following manner permits detection of ES cell germline transmission in a single step by coat color, with NO genotyping required!

Additionally, the germline progeny are identical to the C57BL/6 genome at all coat color loci!


Color:

Albino

Species:

Mouse

Initial Publication:

Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G. (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 2014 Mar 5;9(3):e90570.   



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.