huNOG-EXL Model Portfolio
HUMANIZED IMMUNE SYSTEM MICE

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A Humanized Immune System Mouse Supporting Both Myeloid and Lymphoid Cells

The huNOG-EXL supports superior levels of human cell engraftment and the differentiation of both human myeloid and lymphoid cells. It also offers a longer average lifespan compared to competing myeloid-supportive humanized immune system (HIS) models. Taconic Biosciences' portfolio of HIS mice includes the huNOG-EXL SA (Standard Access) model and the huNOG-EXL EA (Early Access) model.

Each model supports different types of studies. The SA model is shipped to the customer after a quality control step at 10 weeks post-engraftment. However, research applications requiring particularly long study timelines or importation into regions with long quarantine periods present experimental and logistical hurdles that can limit the utility of this version of the huNOG-EXL.

The EA model removes those challenges by providing access to huNOG-EXL mice soon after engraftment, extending the useful study window. This makes the huNOG-EXL EA ideal for engraftment of slow-growing tumors, longer treatment paradigms, or various study customizations.

The huNOG-EXL SA and EA models are available with simple terms of use for academia, pharma, biotech, and CROs, including use in academia-industry collaborations and sponsored or contract studies. Expert support from Taconic's Field Applications Scientists (FAS) plus data from collaborations and R&D across a range of therapeutic areas can enable the successful application of the huNOG-EXL in your research program.

WHICH huNOG-EXL VERSION IS RIGHT FOR YOU?

huNOG-EXL SA
STANDARD ACCESS


Animals ship following human immune cell
reconstitution

  • For researchers who want access to live inventory for immediate delivery, the huNOG-EXL SA is the ideal approach, particularly for experiments with shorter timelines or studies that must start as soon as possible.

huNOG-EXL EA
EARLY ACCESS


Animals ship prior to human immune cell
reconstitution

  • For researchers with certain experimental designs or logistical hurdles, early access to huNOG-EXL mice soon after engraftment may be advantageous, particularly for longer study timelines which may push the bounds of the useful study window in these mice.

WHAT ARE THE KEY DISTINCTIONS BETWEEN THESE huNOG-EXL MODELS?

huNOG-EXL SA

  • Ships after human immune cell chimerism is established in the periphery
  • Live inventory available for immediate delivery
  • Shorter study window once received by the customer
  • Best suited for shorter experiments
  • Live inventory at a range of post-engraftment timepoints allows selection of animals with T cells established for quick start of T cell-relevant studies
  • Most suitable when quarantine is not required
  • All mice shipped meet a specified QC standard of has ≥25% hCD45 in peripheral blood at 10 weeks post-engraftment
  • Taconic's QC process screens out failed lots and individual animals which fail to engraft

huNOG-EXL EA

  • Ships prior to human immune cell reconstitution in the periphery
  • Made to order and shipped within 2 weeks post-engraftment
  • Maximizes potential study window
  • Ideal for engraftment of slow growing tumors, longer treatment paradigms, or various study customizations
  • Allows experimental start prior to emergence of T cells or during the peak B cell window
  • Good choice when a long quarantine period is required
  • Shipped without flow cytometry QC by Taconic, allowing the customer to perform QC relevant to specific experimental design (timing and markers)
  • While lot failures are expected to be rare, individual mice may fail to develop significant human chimerism

huNOG-EXL MODEL TIMELINE COMPARISON

Both versions of the huNOG-EXL start with engraftment in Taconic's humanization lab by highly experienced technicians. The same engraftment protocol is used for both huNOG-EXL SA and huNOG-EXL EA mice, with hematopoietic stem cells (HSCs) for EA engraftments chosen from donors with proven successful engraftment. huNOG-EXL EA mice ship out to customers within 2 weeks post-engraftment (WPE), whereas huNOG-EXL SA mice stay in the humanization lab through the 10 WPE QC check and ship to customers starting at 11-12 WPE.


huNOG-EXL Model Timeline Comparison

Both versions of the huNOG-EXL start with engraftment in Taconic's humanization lab by highly experienced technicians. The same engraftment protocol is used for both huNOG-EXL SA and huNOG-EXL EA mice, with hematopoietic stem cells (HSCs) for Early Access engraftments chosen from donors with proven successful engraftment. huNOG-EXL EA mice ship out to customers within 2 weeks post-engraftment (WPE), whereas huNOG-EXL SA mice stay in the humanization lab through the 10 WPE QC check and ship to customers starting at 11 WPE.

LIFESPAN IN MYELOID-SUPPORTIVE HIS MICE

All myeloid-supportive HIS mice have limited lifespans due to a range of outcomes including anemia, thrombocytopenia, macrophage activation syndrome, and hemophagocytic lymphohistiocytosis. The huNOG-EXL has the longest demonstrated lifespan compared to competing models, but this varies by donor and can be impacted by environmental and experimental factors. Taconic has observed that huNOG-EXL mice can often survive for 30+ WPE, so researchers should plan for a typical study window of 26-30 WPE. Contact us for additional guidance on HIS model-specific housing and husbandry.

KINETICS OF HUMAN IMMUNE CELL RECONSTITUTION IN huNOG-EXL (PERIPHERAL BLOOD)

Kinetics of Human Immune Cell Reconstitution in huNOG-EXL (Peripheral Blood)

huNOG-EXL mice support high levels of human immune cells in peripheral blood (chimerism) and develop both myeloid and lymphoid cells. huNOG-EXL EA mice are shipped shortly after engraftment, prior to reconstitution of significant numbers of human immune cells in peripheral blood. huNOG-EXL SA mice are shipped after a QC step to confirm that each mouse has ≥25% human cells in peripheral blood at 10 weeks post-engraftment.

HOW TACONIC ADDRESSES HIS MOUSE VARIABILITY

Donor characteristics impact human immune system reconstitution in huNOG-EXL mice, including chimerism levels and frequencies of specific human immune cell types. As shown in the graph of huNOG-EXL SA models below, engraftment lots for which the mean falls below Taconic's 25% chimerism threshold are disqualified from sale (shown in red). For lots with a mean above the threshold (shown in blue), any individual animals in the lot which score below 25% are disqualified from sale. For huNOG-EXL EA, Taconic selects donors that have previously been validated to reduce the risk of lot failure. Taconic's Field Application Scientists can help you design a successful study plan which appropriately accounts for both inter- and intra-donor variation in HIS mice and select the right huNOG-EXL version for your research.

How Taconic Addresses HIS Mouse Variability

huNOG-EXL EA MICE ARE ENGRAFTED USING DONOR CELLS WITH PROVEN ENGRAFTMENT SUCCESS

huNOG-EXL EA Mice are Engrafted Using Donor Cells with Proven Engraftment Success

In order to determine the level of engraftment in huNOG-EXL models, HSCs from the same donor were engrafted twice, on separate days, to generate two lots of huNOG-EXL mice per donor. The ability of a donor to produce lots where most mice either met or failed the QC threshold of ≥25% chimerism was replicated between duplicate lots, with one exception in this test set. To generate huNOG-EXL EA mice, Taconic chooses donors with proven engraftment success in order to reduce the risk of lot failure.


Hematopoiesis and Humanized Immune System Mice

Hematopoiesis and Humanized Immune System Mice

Both huNOG-EXL SA and EA models develop human myeloid and lymphoid lineages.

Contact Taconic for more information on the presence and functionality of your cell type of interest.

FEATURED huNOG-EXL MODEL RESOURCES

  • Preclinical Immuno-oncology Models: Humanized Immune System Mice and Beyond

    Virtual Workshop

    Preclinical Immuno-oncology Models: Humanized Immune System Mice and Beyond

  • Three Methods for Generating Humanized Mice

    Insight

    Important Differences in Human Cell Frequencies, Cytokine Profiles, and Survival between Humanized NOG-EXL and NSG-SGM3 Mice

  • Immuno-Oncology Applications of Myeloid-Supportive Humanized Immune System Mice

    On-Demand Webinar

    Immuno-Oncology Applications of Myeloid-Supportive Humanized Immune System Mice

  • Humanized Mouse Models for Drug Discovery: The NOG Portfolio

    White Paper

    Humanized Mouse Models for Drug Discovery: The NOG Portfolio

FREQUENTLY ASKED QUESTIONS (FAQs) ABOUT huNOG-EXL MODELS

The huNOG-EXL EA (Early Access) model is better suited for a longer study such as those with slow-growing patient-derived xenografts or tumor cell lines. Both huNOG-EXL models have a limited lifespan (as with all myeloid-supportive HIS models), but utilizing the huNOG EA allows research studies to begin sooner, thereby accommodating studies that take more time.
Blood sampling can negatively affect the health and well-being of huNOG-EXL mice. To mitigate this, Taconic recommends that blood sampling be limited to once in a two-week period in both huNOG-EXL EA and SA models. Taconic's FAS are available for consultation regarding sampling questions in these models.
Yes. Both huNOG-EXL EA and SA models require special housing and husbandry conditions, as well as proper acclimation and enrichment. Taconic has composed a comprehensive document for customers looking to order a humanized model. Please review Licensing, Care & Resources for Taconic's Humanized Immune System Models and Video: Care of HIS mice for experimental success prior to ordering.
The huNOG-EXL EA and SA models both support the differentiation of multiple human lineages including T, B, and NK cells and various myeloid cells. While not all human immune cell types have been exhaustively characterized in these mice, extensive myeloid differentiation including granulocyte differentiation (basophil, neutrophil and mast cells) are evident in blood and tissues. Antigen presenting cells (dendritic cells and macrophages) are also increased in frequency, both in blood and spleen. Consultation with a Taconic FAS can help you design a project that studies specific cell type(s) of interest in a huNOG-EXL or recommend another model, if more appropriate for the relevant cell type.

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