The huNOG-EXL supports superior levels of human cell engraftment and the differentiation of both human myeloid and lymphoid cells. It also offers a longer average lifespan compared to competing myeloid-supportive humanized immune system (HIS) models. Taconic Biosciences' portfolio of HIS mice includes the huNOG-EXL SA (Standard Access) model and the huNOG-EXL EA (Early Access) model.
Each model supports different types of studies. The SA model is shipped to the customer after a quality control step at 10 weeks post-engraftment. However, research applications requiring particularly long study timelines or importation into regions with long quarantine periods present experimental and logistical hurdles that can limit the utility of this version of the huNOG-EXL.
The EA model removes those challenges by providing access to huNOG-EXL mice soon after engraftment, extending the useful study window. This makes the huNOG-EXL EA ideal for engraftment of slow-growing tumors, longer treatment paradigms, or various study customizations.
The huNOG-EXL SA and EA models are available with simple terms of use for academia, pharma, biotech, and CROs, including use in academia-industry collaborations and sponsored or contract studies. Expert support from Taconic's Field Applications Scientists (FAS) plus data from collaborations and R&D across a range of therapeutic areas can enable the successful application of the huNOG-EXL in your research program.
Both versions of the huNOG-EXL start with engraftment in Taconic's humanization lab by highly experienced technicians. The same engraftment protocol is used for both huNOG-EXL SA and huNOG-EXL EA mice, with hematopoietic stem cells (HSCs) for EA engraftments chosen from donors with proven successful engraftment. huNOG-EXL EA mice ship out to customers within 2 weeks post-engraftment (WPE), whereas huNOG-EXL SA mice stay in the humanization lab through the 10 WPE QC check and ship to customers starting at 11-12 WPE.
Both versions of the huNOG-EXL start with engraftment in Taconic's humanization lab by highly experienced technicians. The same engraftment protocol is used for both huNOG-EXL SA and huNOG-EXL EA mice, with hematopoietic stem cells (HSCs) for Early Access engraftments chosen from donors with proven successful engraftment. huNOG-EXL EA mice ship out to customers within 2 weeks post-engraftment (WPE), whereas huNOG-EXL SA mice stay in the humanization lab through the 10 WPE QC check and ship to customers starting at 11 WPE.
All myeloid-supportive HIS mice have limited lifespans due to a range of outcomes including anemia, thrombocytopenia, macrophage activation syndrome, and hemophagocytic lymphohistiocytosis. The huNOG-EXL has the longest demonstrated lifespan compared to competing models, but this varies by donor and can be impacted by environmental and experimental factors. Taconic has observed that huNOG-EXL mice can often survive for 30+ WPE, so researchers should plan for a typical study window of 26-30 WPE. Contact us for additional guidance on HIS model-specific housing and husbandry.
huNOG-EXL mice support high levels of human immune cells in peripheral blood (chimerism) and develop both myeloid and lymphoid cells. huNOG-EXL EA mice are shipped shortly after engraftment, prior to reconstitution of significant numbers of human immune cells in peripheral blood. huNOG-EXL SA mice are shipped after a QC step to confirm that each mouse has ≥25% human cells in peripheral blood at 10 weeks post-engraftment.
Donor characteristics impact human immune system reconstitution in huNOG-EXL mice, including chimerism levels and frequencies of specific human immune cell types. As shown in the graph of huNOG-EXL SA models below, engraftment lots for which the mean falls below Taconic's 25% chimerism threshold are disqualified from sale (shown in red). For lots with a mean above the threshold (shown in blue), any individual animals in the lot which score below 25% are disqualified from sale. For huNOG-EXL EA, Taconic selects donors that have previously been validated to reduce the risk of lot failure. Taconic's Field Application Scientists can help you design a successful study plan which appropriately accounts for both inter- and intra-donor variation in HIS mice and select the right huNOG-EXL version for your research.
In order to determine the level of engraftment in huNOG-EXL models, HSCs from the same donor were engrafted twice, on separate days, to generate two lots of huNOG-EXL mice per donor. The ability of a donor to produce lots where most mice either met or failed the QC threshold of ≥25% chimerism was replicated between duplicate lots, with one exception in this test set. To generate huNOG-EXL EA mice, Taconic chooses donors with proven engraftment success in order to reduce the risk of lot failure.
Both huNOG-EXL SA and EA models develop human myeloid and lymphoid lineages.
Contact Taconic for more information on the presence and functionality of your cell type of interest.
Preclinical Immuno-oncology Models: Humanized Immune System Mice and Beyond
Immuno-Oncology Applications of Myeloid-Supportive Humanized Immune System Mice
Important Differences in Human Cell Frequencies, Cytokine Profiles, and Survival between Humanized NOG-EXL and NSG-SGM3 Mice
huNOG-EXL EA (Early Access): A Myeloid-Supportive HIS Model with a Longer Potential Study Window
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