PXR

Constitutive Knockout

PXR Constitutive Knockout Mouse Model
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CALL: 888.822.6642 + 45 70 23 04 05 (EUROPE)
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 1414-F
    ko/ko
    B6.129-Nr1i2tm1Sakl N10
  • 1414-M
    ko/ko
    B6.129-Nr1i2tm1Sakl N10

  • Carries a disruption of the nuclear receptor gene Nr1i2, which encodes the pregnane X receptor (PXR)
  • Useful for studies of xenobiotic metabolism
  • Useful for studies of cholestasis and hepatotoxicity

Genetic Background:

C57BL/6 Background

Origin:

The PXR mouse was developed in the laboratory of Steven A. Kliewer at GlaxoSmithKline. The model was created by targeting the Nr1i2 gene in R1 embryonic stem cells derived from 129 mice and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to C57BL/6J for two generations. Taconic received stock in 2000. The line was embryo transfer derived and backcrossed to C57BL/6NTac for a total of 10 generations (N10). The line was rederived in 2005. The colony is maintained through incrossing of homozygous mice.


Availability:

Occasionally, it becomes necessary to discontinue live production of Taconic Transgenic Models™ due to lack of interest. The PXR mouse developed in the laboratory of Steven A. Kliewer at GlaxoSmithKline is one such model. Live production of the model ended on May 15, 2013. If you have a need for a Pxr knockout mouse model, please contact us.

Color:

Black

Species:

Mouse

Initial Publication:

Staudinger JL, Goodwin B, Jones SA, Hawkins-Brown D, MacKenzie KI, LaTour A, Liu Y, Klaassen CD, Brown KK, Reinhard J, Willson TM, Koller BH, Kliewer SA. (2001) The nuclear receptor PXR is a lithocholic acid sensor that protects against liver toxicity. Proc Natl Acad Sci USA 98(6):3369-74.



Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.