The PXR mouse was developed in the laboratory of Steven A. Kliewer at GlaxoSmithKline. The model was created by targeting the Nr1i2 gene in R1 embryonic stem cells derived from 129 mice and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to C57BL/6J for two generations. Taconic received stock in 2000. The line was embryo transfer derived and backcrossed to C57BL/6NTac for a total of 10 generations (N10). The line was rederived in 2005. The colony is maintained through incrossing of homozygous mice.
Staudinger JL, Goodwin B, Jones SA, Hawkins-Brown D, MacKenzie KI, LaTour A, Liu Y, Klaassen CD, Brown KK, Reinhard J, Willson TM, Koller BH, Kliewer SA. (2001) The nuclear receptor PXR is a lithocholic acid sensor that protects against liver toxicity. Proc Natl Acad Sci USA 98(6):3369-74.