Orders by weight:
This triple targeted mutation model carries a disruption of the multi-drug resistance genes Abcb1a (encoding p-glycoprotein 3), Abcb1b (encoding p-glycoprotein 1) and Abcg2 (encoding BCRP/ABCG2)
Useful in studies of drug transport, oral bioavailability and multi-drug resistance
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background: FVB Background
Origin: The Mdr1a/b-Bcrp mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created through crossbreeding of the Mdr1a/b targeted mutation mouse and the Bcrp targeted mutation mouse in the Schinkel lab. The Mdr1a/b model was created through sequential targeting of the Abcb1a and Abcb1b genes in E14 ES cells. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). The Bcrp model was created by targeting the Abcg2 gene in E14 embryonic stem cells derived from 129P2/OlaHsd mice and injecting the targeted cells into FVB blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). Taconic received stock of the triple targeted mutation line in April 2005. The colony is maintained by mating animals homozygous for all three mutations.
Genetics: Wild type for Nnt mutation; carries Pde6brd1 mutation
Jonker JW, Freeman J, Bolscher E, Musters S, Alvi AJ, Titley I, Schinkel AH, Dale TC (2005). Contribution of the ABC transporters Bcrp1 and Mdr1a/1b to the side population phenotype in mammary gland and bone marrow of mice. Stem Cells, 23(8):1059-1065