Mdr1a/b-Bcrp

Constitutive Knockout

Mdr1a/b-Bcrp Constitutive Knockout Mouse Model
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FVB Background

  • Model #
  • Genotype
  • Nomenclature
  • 3998-F
    ko/ko;ko/ko;ko/ko
    FVB.129P2-Abcb1atm1Bor Abcb1btm1Bor Abcg2tm1Ahs N7
  • 3998-M
    ko/ko;ko/ko;ko/ko
    FVB.129P2-Abcb1atm1Bor Abcb1btm1Bor Abcg2tm1Ahs N7

  • This triple targeted mutation model carries a disruption of the multi-drug resistance genes Abcb1a (encoding p-glycoprotein 3), Abcb1b (encoding p-glycoprotein 1) and Abcg2 (encoding BCRP/ABCG2)
  • Useful in studies of drug transport, oral bioavailability and multi-drug resistance
  • Triple homozygote mice may develop phototoxicity upon exposure to pheophorbide a, a chlorophyll metabolite, first expressed as ear lesions and can progress to mice becoming moribund. They may also display a novel type of protoporphyria characterized by elevated levels of protoporphrin IX. Both of these effects have been observed in single gene Bcrp homozygous knockouts.
  • See additional information on the roles of drug transporters Bcrp, Mdr1a and Mdr1b.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

FVB Background

Origin:

The Mdr1a/b-Bcrp mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created through crossbreeding of the Mdr1a/b targeted mutation mouse and the Bcrp targeted mutation mouse in the Schinkel lab. The Mdr1a/b model was created through sequential targeting of the Abcb1a and Abcb1b genes in E14 ES cells. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). The Bcrp model was created by targeting the Abcg2 gene in E14 embryonic stem cells derived from 129P2/OlaHsd mice and injecting the targeted cells into FVB blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). Taconic received stock of the triple targeted mutation line in April 2005. The colony is maintained by mating animals homozygous for all three mutations.


Genetics:

Wild type for Nnt mutation; carries Pde6brd1 mutation


Color:

Albino

Species:

Mouse

Initial Publication:

Jonker JW, Freeman J, Bolscher E, Musters S, Alvi AJ, Titley I, Schinkel AH, Dale TC (2005). Contribution of the ABC transporters Bcrp1 and Mdr1a/1b to the side population phenotype in mammary gland and bone marrow of mice. Stem Cells, 23(8):1059-1065.



Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
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