The LTH-1 transgenic mouse was established by N. Labrecque et al. at the Insitut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM/ULP in Strasbourg, France. The tTA coding region was excised from the plasmid of pUHD15-1, subcloned in pBlueScript and a stretch encoding tTA was removed as a SpeI-EcoRV fragment and blunted. This fragment was then cloned into the filled-in BamHI site of plasmid p1017 containing the Lck proximal promoter and human growth hormone intron/exon and polyadenylation sequences. A 6.4 kb NotI fragment of the Lck-tTA was microinjected into B6SJLF2 fertilized eggs and the transgene was identified by PCR. Mice that had been backcrossed 8 times to C57BL/6NTac were transferred to the NIAID repository at Taconic in 2002. After expansion, N9 donors were embryo rederived to C57BL/6NTac females. Subsequent heterozygotes were identified by PCR and intracrossed. The N10 homozygous mice were identified by progeny testing.
Labrecque N, Whitfield LS, Obst R, Waltzinger C. Benoist C, and Mathis D. How much TCR does a T cell need? Immunity 2001; 15:71-82.