LTH-1 Transgenic

Random Transgenic

LTH-1 Random Transgenic Mouse Model
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The NIAID Exchange Program has closed and live production of this model has ceased. This webpage is retained for historical reference, but this model is not available from Taconic.

  • Model #
  • Genotype
  • Nomenclature
  • 4245-F
    B6.SJL-Tg(Lck-tTA)1Dim N10
  • 4245-M
    B6.SJL-Tg(Lck-tTA)1Dim N10

The C57BL/6-TgN(LTH-1) line is carrier for a transgene expressing an lck proximal promoter driving a tetracycline-sensitive TetR-VP16 transactivator (lck-tTA). The lck-tTA vector is active only in lymphoid organs, except for a weak signal in the ovaries. More precisely, the transcripts are localized to T lineage cells. There is a strong expression in thymocyctes starting at the earliest double negative stage, and a weaker expression in peripheral T cells. The TgN(LTH-1) mouse can be mated to other transgenic mice containing cDNA(s) of interest under the control of a (minimal) promoter augmented by several TeT-O sequences. Thus, the expression of tTA in T cells will turn on transcription of the second cDNA at the same time as the expression of lck and this transactivation is susceptible to inhibition by tetracycline in a temporal and quantitative fashion.

Genetic Background:

C57BL/6 Background

Origin:

The LTH-1 transgenic mouse was established by N. Labrecque et al. at the Insitut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM/ULP in Strasbourg, France. The tTA coding region was excised from the plasmid of pUHD15-1, subcloned in pBlueScript and a stretch encoding tTA was removed as a SpeI-EcoRV fragment and blunted. This fragment was then cloned into the filled-in BamHI site of plasmid p1017 containing the Lck proximal promoter and human growth hormone intron/exon and polyadenylation sequences. A 6.4 kb NotI fragment of the Lck-tTA was microinjected into B6SJLF2 fertilized eggs and the transgene was identified by PCR. Mice that had been backcrossed 8 times to C57BL/6NTac were transferred to the NIAID repository at Taconic in 2002. After expansion, N9 donors were embryo rederived to C57BL/6NTac females. Subsequent heterozygotes were identified by PCR and intracrossed. The N10 homozygous mice were identified by progeny testing.


Availability:

This model is no longer available.

Color:

Black

Species:

Mouse

Initial Publication:

Labrecque N, Whitfield LS, Obst R, Waltzinger C. Benoist C, and Mathis D. How much TCR does a T cell need? Immunity 2001; 15:71-82.


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