The Transgenic DO11.10 T Cell Receptor/Transgenic CARΔ line was made by James DeGregori at the University of Colorado Health Science Center in 2000. A human CARΔ-I cDNA was cloned into the AscI-digested lckB proximal promoter/human CD2 enhancer transgene construct. CARΔ-I transgenic mice were then generated by pronuclear microinjection of this DNA construct into FVB/NJ zygotes The CAR positive mice were maintained by backcrossing onto BALB/cJ for greater than 10 generations. The CAR transgenic mice to were bred to BALB/cJ mice containing the DO11.10 transgene (created by K. Murphy and D. Loh). These mice were subsequently maintained by intracrossing for several generations. The double transgenic mice were transferred to the NIAID repository at Taconic in 2004 and after expansion, mice which tested positive for both Tgs using flow cytometry, were embryo transfer derived. These mice were then intracrossed and mice homozygous for both the TCR DO11.10 and CARΔ-I transgenes were identified by test mating the mice to wild type partners and selecting animals that only gave rise to double transgene positive offspring.
Leon RP, Hedlund T, Meech SJ, Li S, Schaack J, Hunger SP, Duke RC, DeGregori J: Adenoviral-mediated gene transfer in lymphocytes. PNAS USA vol. 95, pp. 13519-13164 1998.
Wan YY, Leon RP, Marks R, Cham CM, Schaack J, Gajewski TF, DeGregori J: Transgenic expression of the coxsackie/adenovirus receptor enables adenoviral-mediated gene delivery in naïve T cells. PNAS USA vol. 97, pp. 13784-13789 2000.
Wan YY and DeGregori J: The survival of antigen-stimulated T cells require NFkB-mediated inhibition of p73 expression. Immunity vol. 18, pp. 331-342 2003.
Murphy KM, Heimberger, and Loh DY: Induction by antigen of intrathymic apoptosis of CD4+ CD8+ TCRlo thymocytes in vivo. Science 250, pp. 1720-1723 1990
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