Mouse Bioassay Identifies Botulism Toxin
In the mouse botulism bioassay, patient samples are injected intraperitoneally. The presence of botulinum toxin results in rapid development of disease symptoms, such as muscle weakness and respiratory failure, typically within one-to-five days. This assay can also be used to identify which of the seven botulism toxin types are present.
Mice are treated with one of the seven antitoxins, then challenged with a patient sample which has been proven toxic in mice. By observing which mice are protected from sickness by a particular antitoxin, the assay permits identification of the toxin type.
In the case of the Danish outbreak, the mouse assay confirmed the presence of botulism toxin the blood of the affected people, with results coming as quickly as four hours after sample injection.