IHMS™ Testing Program

Testing frequency, methodology and sample number is based on an assessment of biosecurity and risk level and varies depending on whether the location uses static or individually ventilated caging (IVC) or is isolator-raised. Taconic Biosciences is transitioning all RF™, OF™ and EF locations to IVCs. IVCs are more biosecure, but pose some diagnostic challenges, which Taconic addresses through additional testing elements. Taconic has designed a multifaceted testing program in which agents of highest concern are screened using different methodologies to increase the likelihood of accurate and rapid detection.

International Health Monitoring System™

Core testing program Opportunist screening IVC PCR testing IVC exhaust air duct testing Isolator microbial monitoring 16S bacterial RNA screening
Predominantly sentinel-based health testing comprised of virus detection, full bacteriology and parasitology. Core testing occurs in ALL Taconic production locations. Agents are typically tested quarterly in gnotobiotic isolators (GF/DF standards) and IVC locations (some RF, OF and EF colonies) or monthly (static cage RF, OF, EF colonies), but this varies by agent. Click for full details on test method and frequency. Monthly culture-based screening to detect non-tolerated opportunists at the RF, OF and EF standards (aerobic culture). Click for full details on agents tested and test methods. Additional cage level testing of line animals via PCR in locations using IVC housing. This added screening uses a different test methodology from the core testing program to enhance the overall detectability of key agents and is designed to address the challenge of animal health monitoring in IVCs. Click for full details on agents tested and frequency. Screening of the exhaust air from IVC cages to provide a snapshot of all cages on a rack. This method is particularly well suited to detection of certain agents. Click for full details on agents tested and frequency. Culture-based screening to detect bacterial contamination of gnotobiotic isolators (aerobic and anaerobic culture). Click for full details on test method and frequency. 16S PCR screening in Germ Free isolators to detect bacterial RNA. Testing is performed on pooled fecal samples from each isolator on a monthly basis.

Core testing program

The core testing program is comprised of virus detection, full bacteriology and parasitology. This testing predominantly uses sentinels, but in locations with immunodeficient animals, line animals may be used for the diagnostic portion of health monitoring (microbiology, molecular biology, and parasitology), with sentinels used for the serological portion. For all barrier housing units with static caging: Quarterly, Semiannual and Annual core panels test a minimum of 10 animals, and Monthly core panels test a minimum of 5 animals. For housing units with IVCs: Quarterly, Semiannual and Annual core panels test 1 animal per housing rack side. Additional cage level PCR and exhaust air duct (EAD) testing is performed in quarterly in IVC locations. In IVC locations, the three test programs (core testing, IVC PCR and exhaust air duct testing) rotate such that testing for one of the three program elements occurs each month.

Agents Species Tested Test Method EF/OF/RF™/MPF™ Static Cage Frequency EF/OF Individually Ventilaged Cage Frequency GF/DF Frequency

Viruses

Kilham Rat Virus (KRV) R MFI Monthly Quarterly Quarterly
Minute Virus of Mice (MVM) M MFI Monthly Quarterly Quarterly
Mouse Encephalomyelitis Virus (GDVII)/ Rat Theilovirus (RTV) M, R MFI Monthly Quarterly Quarterly
Mouse Hepatitis Virus (MHV) M MFI Monthly Quarterly Quarterly
Murine Norovirus (MNV) M MFI Monthly Quarterly Quarterly
Mouse Parvovirus (MPV) M MFI Monthly Quarterly Quarterly
Mouse Rotavirus (EDIM) M MFI Monthly Quarterly Quarterly
Pneumonia Virus (PVM) M, R MFI Monthly Quarterly Quarterly
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R MFI Monthly Quarterly Quarterly
Rat Minute Virus (RMV) R MFI Monthly Quarterly Quarterly
Rat Parvovirus (RPV) R MFI Monthly Quarterly Quarterly
Toolan's H1 Virus (TH1) R MFI Monthly Quarterly Quarterly
Ectromelia Virus M MFI Quarterly Quarterly Quarterly
Hantaan Virus M, R MFI Quarterly Quarterly Quarterly
K Virus M MFI Quarterly Quarterly Quarterly
Lymphocytic Choriomeningitis Virus (LCM) M, R MFI Quarterly Quarterly Quarterly
Mouse Adenovirus I and II (FL and K87) M, R MFI Quarterly Quarterly Quarterly
Mouse Cytomegalovirus (MCMV) M MFI Quarterly Quarterly Quarterly
Polyoma Virus M MFI Quarterly Quarterly Quarterly
Reovirus (Reo 3) M, R MFI Quarterly Quarterly Quarterly
Sendai Virus M, R MFI Quarterly Quarterly Quarterly
Thymic Virus M IFA Semiannually Semiannually Semiannually
Lactate Dehydrogenase Elevating Virus (LDHV) M MFI Annually Annually Annually

Bacteria, mycoplasma, fungi

Beta hemolytic Streptococcus (non Group D) M, R Culture/PCR Quarterly Quarterly Quarterly
Bordetella bronchiseptica M, R Culture/PCR Quarterly Quarterly Quarterly
Cilia Associated Respiratory Bacillus (CARB) M, R MFI Quarterly Quarterly Quarterly
Citrobacter rodentium M Culture/PCR Quarterly Quarterly Quarterly
Clostridium piliforme M, R MFI Quarterly Quarterly Quarterly
Corynebacterium bovis1 M, R Culture/PCR Quarterly Quarterly Quarterly
Corynebacterium kutscheri M, R Culture/PCR Quarterly Quarterly Quarterly
Helicobacter spp. M, R PCR Quarterly Quarterly Quarterly
Klebsiella oxytoca M, R Culture/PCR Quarterly Quarterly Quarterly
Klebsiella pneumoniae M, R Culture/PCR Quarterly Quarterly Quarterly
Mycoplasma pulmonis M, R MFI Quarterly Quarterly Quarterly
Pasteurella multocida M, R PCR Quarterly Quarterly Quarterly
Pasteurella pneumotropica M, R Culture/PCR Quarterly Quarterly Quarterly
Pneumocystis spp. M, R PCR Quarterly Quarterly Quarterly
Proteus spp. M, R Culture/PCR Quarterly Quarterly Quarterly
Pseudomonas aeruginosa M, R Culture/PCR Quarterly Quarterly Quarterly
Salmonella spp. M, R Culture/PCR Quarterly Quarterly Quarterly
Staphylococcus aureus M, R Culture/PCR Quarterly Quarterly Quarterly
Streptococcus pneumoniae M, R Culture/PCR Quarterly Quarterly Quarterly
Segmented Filamentous Bacteria (SFB) M, R PCR Annually Annually Annually
Streptobacillus moniliformis M, R PCR Annually Annually Annually

Parasites

Aspiculuris tetraptera M, R Microscopy/PCR Quarterly Quarterly Quarterly
Eimeria spp. M, R Microscopy/PCR Quarterly Quarterly Quarterly
Encephalitozoon cuniculi M, R MFI Quarterly Quarterly Quarterly
Entamoeba spp. M, R Microscopy/PCR Quarterly Quarterly Quarterly
Giardia muris M, R Microscopy/PCR Quarterly Quarterly Quarterly
Hymenolepis spp. M, R Microscopy/PCR Quarterly Quarterly Quarterly
Myobia musculi M, R Microscopy/PCR Quarterly Quarterly Quarterly
Myocoptes musculinus M, R Microscopy/PCR Quarterly Quarterly Quarterly
Ornithonyssus bacoti M, R Microscopy Quarterly Quarterly Quarterly
Polyplax spinulosa M, R Microscopy Quarterly Quarterly Quarterly
Psorergates simplex M, R Microscopy Quarterly Quarterly Quarterly
Radfordia affinis M, R Microscopy/PCR Quarterly Quarterly Quarterly
Rodentolepis spp. M, R Microscopy/PCR Quarterly Quarterly Quarterly
Spironucleus muris M, R Microscopy/PCR Quarterly Quarterly Quarterly
Syphacia spp. M, R Microscopy/PCR Quarterly Quarterly Quarterly
Trichomonads M, R Microscopy/PCR Quarterly Quarterly Quarterly
1. Testing not performed in MPF colonies

Opportunist screening

Opportunist screening (RF, OF, EF) Sample frequency Source of microbial samples Number of samples
RF: Aerobic cultures for beta hemolytic Streptococci, K. oxytoca, K. pneumoniae, P. aeruginosa, S. aureus, P. multocida
OF: Aerobic cultures for beta hemolytic Streptococci, K. oxytoca, K. pneumoniae, P. aeruginosa, S. aureus, P. multocida, Proteus spp.
EF: Aerobic cultures for beta hemolytic Streptococci, K. oxytoca, K. pneumoniae, P. aeruginosa, S. aureus, P. multocida, Proteus spp. and PCR screening for Segmented Filamentous Bacteria (SFB)
Monthly Fecal pellets and oral swabs of animals For locations with static caging: four random cages per housing rack (fecal only) and all sentinel cages (fecal and oral). For locations with individually ventilated cages: 2 columns per housing rack (fecal only) and all sentinel cages (fecal and oral).

IVC PCR testing

PCR testing is performed on fecal samples collected from line animal cages on IVC racks. 10 line animal cages per rack side are tested on a quarterly basis.

Agents Species IVC PCR (mouse) IVC PCR (rat)
Kilham Rats Virus (KRV) R   X
Minute Virus of Mice (MVM) M X  
Mouse Encephalomyelitis Virus (GDVII)/Rat Theilovirus (RTV) M,R X X
Mouse Hepatitis Virus (MHV) M X  
Mouse Parvovirus (MPV) M X  
Mouse Rotavirus (EDIM) M X  
Murine Norovirus (MNV) M X  
Pneumonia Virus of Mice (PVM) M,R X X
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R   X
Rat Minute Virus (RMV) R   X
Rat Parvovirus (RPV) R   X
Toolan's H1 Virus (TH1) R   X
Corynebacterium bovis M,R X X
Helicobacter spp. M,R X X
Pasteurella multocida M,R X X
Pasteurella pneumotropica M,R X X
Segmented Filamentous Bacteria M,R X X
Aspiculuris tetraptera M,R X X
Entamoeba muris SPECIES M,R X X
Spironucleus muris M,R X X
Syphacia spp. M,R X X
Trichomonads M,R X X

IVC exhaust air duct testing

PCR testing is performed on total nucleic acids extracted from the exhaust air duct (EAD) filter on each IVC rack. This testing is performed quarterly on each rack.

Agents Species EAD (mouse) EAD (rat)
Kilham Rats Virus (KRV) R   X
Minute Virus of Mice (MVM) M X  
Mouse Encephalomyelitis Virus (GDVII)/Rat Theilovirus (RTV) M,R X X
Mouse Hepatitis Virus (MHV) M X  
Mouse Parvovirus (MPV) M X  
Mouse Rotavirus (EDIM) M X  
Murine Norovirus (MNV) M X  
Pneumonia Virus of Mice (PVM) M,R X X
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R   X
Rat Minute Virus (RMV) R   X
Rat Parvovirus (RPV) R   X
Toolan's H1 Virus (TH1) R   X
Corynebacterium bovis M,R X X
Helicobacter spp. M,R X X
Pasteurella multocida M,R X X
Pasteurella pneumotropica M,R X X
Segmented Filamentous Bacteria M,R X X
Aspiculuris tetraptera M,R X X
Entamoeba muris SPECIES M,R X X
Myobia musculi M,R X X
Myocoptes musculinus M,R X X
Ornithonyssus bacoti M,R X X
Spironucleus muris M,R X X
Syphacia spp. M,R X X
Trichomonads M,R X X

Isolator microbial monitoring

Microbial monitoring (DF, GF) Sample frequency Source of microbial samples Number of samples
Cultures for all aerobic and anaerobic organisms Biweekly (DF) or weekly (GF) Animals (feces) and environment (feed, water, isolator surfaces, etc.) One pooled sample per isolator

Health Testing Details by Health Standard

Clicking the links below will show which agents are tested and at what frequency for each health standard.

Murine Pathogen Free™ (MPF™) health standard

    Core testing program Opportunist screening IVC PCR testing IVC exhaust air duct testing Isolator microbial monitoring 16S bacterial RNA screening
Agent Species tested Applicable Not applicable Not applicable Not applicable Not applicable Not applicable
Viruses
Kilham Rat Virus (KRV) R Monthly
Minute Virus of Mice (MVM) M Monthly
Mouse Encephalomyelitis Virus (GDVII)/ Rat Theilovirus (RTV) M, R Monthly
Mouse Hepatitis Virus (MHV) M Monthly
Murine Norovirus (MNV) M Monthly
Mouse Parvovirus (MPV) M Monthly
Mouse Rotavirus (EDIM) M Monthly
Pneumonia Virus(PVM) M, R Monthly
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R Monthly
Rat Minute Virus (RMV) R Monthly
Rat Parvovirus (RPV) R Monthly
Toolan's H1 Virus (TH1) R Monthly
Ectromelia Virus M Quarterly
Hantaan Virus M, R Quarterly
K Virus M Quarterly
Lymphocytic Choriomeningitis Virus (LCM) M, R Quarterly
Mouse Adenovirus I and II (FL and K87) M, R Quarterly
Mouse Cytomegalovirus (MCMV) M Quarterly
Polyoma Virus M Quarterly
Reovirus (Reo 3) M, R Quarterly
Sendai Virus M, R Quarterly
Thymic Virus M Semiannually
Lactate Dehydrogenase Elevating Virus (LDHV) M Annually
Bacteria, mycoplasma, fungi
Beta hemolytic Streptococcus (non Group D) M, R Quarterly
Bordetella bronchiseptica M, R Quarterly
Cilia Associated Respiratory Bacillus (CARB) M, R Quarterly
Citrobacter rodentium M Quarterly
Clostridium piliforme M, R Quarterly
Corynebacterium bovis1 M, R N/A
Corynebacterium kutscheri M, R Quarterly
Helicobacter spp. M, R Quarterly
Klebsiella oxytoca M, R Quarterly
Klebsiella pneumoniae M, R Quarterly
Mycoplasma pulmonis M, R Quarterly
Pasteurella multocida M, R Quarterly
Pasteurella pneumotropica M, R Quarterly
Pneumocystis spp. M, R Quarterly
Proteus spp. M, R Quarterly
Pseudomonas aeruginosa M, R Quarterly
Salmonella spp. M, R Quarterly
Staphylococcus aureus M, R Quarterly
Streptococcus pneumoniae M, R Quarterly
Segmented Filamentous Bacteria(SFB) M, R Annually
Streptobacillus moniliformis M, R Annually
Parasites
Aspiculuris tetraptera M, R Quarterly
Eimeria spp. M, R Quarterly
Encephalitozoon cuniculi M, R Quarterly
Entamoeba spp. M, R Quarterly
Giardia muris M, R Quarterly
Hymenolepis spp. M, R Quarterly
Myobia musculi M, R Quarterly
Myocoptes musculinus M, R Quarterly
Ornithonyssus bacoti M, R Quarterly
Polyplax spinulosa M, R Quarterly
Psorergates simplex M, R Quarterly
Radfordia affinis M, R Quarterly
Rodentolepis spp. M, R Quarterly
Spironucleus muris M, R Quarterly
Syphacia spp. M, R Quarterly
Trichomonads M, R Quarterly
1. Testing not performed in MPF colonies.

Restricted Flora™ (RF™), Opportunist Free™ (OF™) and Excluded Flora (EF) health standards - static caging locations

    Core testing program Opportunist screening IVC PCR testing IVC exhaust air duct testing Isolator microbial monitoring 16S bacterial RNA screening
Agent Species tested Applicable Applicable Not applicable Not applicable Not applicable Not applicable
Viruses
Kilham Rat Virus (KRV) R Monthly
Minute Virus of Mice (MVM) M Monthly
Mouse Encephalomyelitis Virus (GDVII)/ Rat Theilovirus (RTV) M, R Monthly
Mouse Hepatitis Virus (MHV) M Monthly
Murine Norovirus (MNV) M Monthly
Mouse Parvovirus (MPV) M Monthly
Mouse Rotavirus (EDIM) M Monthly
Pneumonia Virus(PVM) M, R Monthly
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R Monthly
Rat Minute Virus (RMV) R Monthly
Rat Parvovirus (RPV) R Monthly
Toolan's H1 Virus (TH1) R Monthly
Ectromelia Virus M Quarterly
Hantaan Virus M, R Quarterly
K Virus M Quarterly
Lymphocytic Choriomeningitis Virus (LCM) M, R Quarterly
Mouse Adenovirus I and II (FL and K87) M, R Quarterly
Mouse Cytomegalovirus (MCMV) M Quarterly
Polyoma Virus M Quarterly
Reovirus (Reo 3) M, R Quarterly
Sendai Virus M, R Quarterly
Thymic Virus M Semiannually
Lactate Dehydrogenase Elevating Virus (LDHV) M Annually
Bacteria, mycoplasma, fungi
Beta hemolytic Streptococcus (non Group D) M, R Quarterly Monthly
Bordetella bronchiseptica M, R Quarterly
Cilia Associated Respiratory Bacillus (CARB) M, R Quarterly
Citrobacter rodentium M Quarterly
Clostridium piliforme M, R Quarterly
Corynebacterium bovis M, R Quarterly
Corynebacterium kutscheri M, R Quarterly
Helicobacter spp. M, R Quarterly
Klebsiella oxytoca M, R Quarterly Monthly
Klebsiella pneumoniae M, R Quarterly Monthly
Mycoplasma pulmonis M, R Quarterly
Pasteurella multocida M, R Quarterly Monthly
Pasteurella pneumotropica M, R Quarterly
Pneumocystis spp. M, R Quarterly
Proteus spp. M, R Quarterly Monthly at OF and EF
Pseudomonas aeruginosa M, R Quarterly Monthly
Salmonella spp. M, R Quarterly
Staphylococcus aureus M, R Quarterly Monthly
Streptococcus pneumoniae M, R Quarterly
Segmented Filamentous Bacteria (SFB) M, R Annually Monthly at EF
Streptobacillus moniliformis M, R Annually
Parasites
Aspiculuris tetraptera M, R Quarterly
Eimeria spp. M, R Quarterly
Encephalitozoon cuniculi M, R Quarterly
Entamoeba spp. M, R Quarterly
Giardia muris M, R Quarterly
Hymenolepis spp. M, R Quarterly
Myobia musculi M, R Quarterly
Myocoptes musculinus M, R Quarterly
Ornithonyssus bacoti M, R Quarterly
Polyplax spinulosa M, R Quarterly
Psorergates simplex M, R Quarterly
Radfordia affinis M, R Quarterly
Rodentolepis spp. M, R Quarterly
Spironucleus muris M, R Quarterly
Syphacia spp. M, R Quarterly
Trichomonads M, R Quarterly

Restricted Flora™ (RF™), Opportunist Free™ (OF™) and Excluded Flora (EF) health standards - individually ventilated caging locations

    Core testing program Opportunist screening IVC PCR testing IVC exhaust air duct testing Isolator microbial monitoring 16S bacterial RNA screening
Agent Species tested Applicable Applicable Applicable Applicable Not applicable Not applicable
Viruses
Kilham Rat Virus (KRV) R Quarterly Quarterly Quarterly
Minute Virus of Mice (MVM) M Quarterly Quarterly Quarterly
Mouse Encephalomyelitis Virus (GDVII)/ Rat Theilovirus (RTV) M, R Quarterly Quarterly Quarterly
Mouse Hepatitis Virus (MHV) M Quarterly Quarterly Quarterly
Murine Norovirus (MNV) M Quarterly Quarterly Quarterly
Mouse Parvovirus (MPV) M Quarterly Quarterly Quarterly
Mouse Rotavirus (EDIM) M Quarterly Quarterly Quarterly
Pneumonia Virus(PVM) M, R Quarterly Quarterly Quarterly
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R Quarterly Quarterly Quarterly
Rat Minute Virus (RMV) R Quarterly Quarterly Quarterly
Rat Parvovirus (RPV) R Quarterly Quarterly Quarterly
Toolan's H1 Virus (TH1) R Quarterly Quarterly Quarterly
Ectromelia Virus M Quarterly
Hantaan Virus M, R Quarterly
K Virus M Quarterly
Lymphocytic Choriomeningitis Virus (LCM) M, R Quarterly
Mouse Adenovirus I and II (FL and K87) M, R Quarterly
Mouse Cytomegalovirus (MCMV) M Quarterly
Polyoma Virus M Quarterly
Reovirus (Reo 3) M, R Quarterly
Sendai Virus M, R Quarterly
Thymic Virus M Semiannually
Lactate Dehydrogenase Elevating Virus (LDHV) M Annually
Bacteria, mycoplasma, fungi
Beta hemolytic Streptococcus (non Group D) M, R Quarterly Monthly
Bordetella bronchiseptica M, R Quarterly
Cilia Associated Respiratory Bacillus (CARB) M, R Quarterly
Citrobacter rodentium M Quarterly
Clostridium piliforme M, R Quarterly
Corynebacterium bovis M, R Quarterly Quarterly Quarterly
Corynebacterium kutscheri M, R Quarterly
Helicobacter spp. M, R Quarterly Quarterly Quarterly
Klebsiella oxytoca M, R Quarterly Monthly
Klebsiella pneumoniae M, R Quarterly Monthly
Mycoplasma pulmonis M, R Quarterly
Pasteurella multocida M, R Quarterly Monthly Quarterly Quarterly
Pasteurella pneumotropica M, R Quarterly Quarterly Quarterly
Pneumocystis spp. M, R Quarterly
Proteus spp. M, R Quarterly Monthly at OF and EF
Pseudomonas aeruginosa M, R Quarterly Monthly
Salmonella spp. M, R Quarterly
Staphylococcus aureus M, R Quarterly Monthly
Streptococcus pneumoniae M, R Quarterly
Segmented Filamentous Bacteria (SFB) M, R Annually Monthly at EF Quarterly Quarterly
Streptobacillus moniliformis M, R Annually
Parasites
Aspiculuris tetraptera M, R Quarterly Quarterly Quarterly
Eimeria spp. M, R Quarterly
Encephalitozoon cuniculi M, R Quarterly
Entamoeba spp. M, R Quarterly Quarterly (Entamoeba muris spp.) Quarterly (Entamoeba muris spp.)
Giardia muris M, R Quarterly
Hymenolepis spp. M, R Quarterly
Myobia musculi M, R Quarterly Quarterly
Myocoptes musculinus M, R Quarterly Quarterly
Ornithonyssus bacoti M, R Quarterly Quarterly
Polyplax spinulosa M, R Quarterly
Psorergates simplex M, R Quarterly
Radfordia affinis M, R Quarterly
Rodentolepis spp. M, R Quarterly
Spironucleus muris M, R Quarterly Quarterly Quarterly
Syphacia spp. M, R Quarterly Quarterly Quarterly
Trichomonads M, R Quarterly Quarterly Quarterly

Defined Flora (DF) health standard

    Core testing program Opportunist screening IVC PCR testing IVC exhaust air duct testing Isolator microbial monitoring 16S bacterial RNA screening
Agent Species tested Applicable Not applicable Not applicable Not applicable Applicable Not applicable
Viruses
Kilham Rat Virus (KRV) R Quarterly
Minute Virus of Mice (MVM) M Quarterly
Mouse Encephalomyelitis Virus (GDVII)/ Rat Theilovirus (RTV) M, R Quarterly
Mouse Hepatitis Virus (MHV) M Quarterly
Murine Norovirus (MNV) M Quarterly
Mouse Parvovirus (MPV) M Quarterly
Mouse Rotavirus (EDIM) M Quarterly
Pneumonia Virus(PVM) M, R Quarterly
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R Quarterly
Rat Minute Virus (RMV) R Quarterly
Rat Parvovirus (RPV) R Quarterly
Toolan's H1 Virus (TH1) R Quarterly
Ectromelia Virus M Quarterly
Hantaan Virus M, R Quarterly
K Virus M Quarterly
Lymphocytic Choriomeningitis Virus (LCM) M, R Quarterly
Mouse Adenovirus I and II (FL and K87) M, R Quarterly
Mouse Cytomegalovirus (MCMV) M Quarterly
Polyoma Virus M Quarterly
Reovirus (Reo 3) M, R Quarterly
Sendai Virus M, R Quarterly
Thymic Virus M Semiannually
Lactate Dehydrogenase Elevating Virus (LDHV) M Annually
Bacteria, mycoplasma, fungi All bacterial, mycoplasma, fungal contaminants monitored bi-weekly
Beta hemolytic Streptococcus (non Group D) M, R Quarterly
Bordetella bronchiseptica M, R Quarterly
Cilia Associated Respiratory Bacillus (CARB) M, R Quarterly
Citrobacter rodentium M Quarterly
Clostridium piliforme M, R Quarterly
Corynebacterium bovis M, R Quarterly
Corynebacterium kutscheri M, R Quarterly
Helicobacter spp. M, R Quarterly
Klebsiella oxytoca M, R Quarterly
Klebsiella pneumoniae M, R Quarterly
Mycoplasma pulmonis M, R Quarterly
Pasteurella multocida M, R Quarterly
Pasteurella pneumotropica M, R Quarterly
Pneumocystis spp. M, R Quarterly
Proteus spp. M, R Quarterly
Pseudomonas aeruginosa M, R Quarterly
Salmonella spp. M, R Quarterly
Staphylococcus aureus M, R Quarterly
Streptococcus pneumoniae M, R Quarterly
Segmented Filamentous Bacteria (SFB) M, R Annually
Streptobacillus moniliformis M, R Annually
Parasites
Aspiculuris tetraptera M, R Quarterly
Eimeria spp. M, R Quarterly
Encephalitozoon cuniculi M, R Quarterly
Entamoeba spp. M, R Quarterly
Giardia muris M, R Quarterly
Hymenolepis spp. M, R Quarterly
Myobia musculi M, R Quarterly
Myocoptes musculinus M, R Quarterly
Ornithonyssus bacoti M, R Quarterly
Polyplax spinulosa M, R Quarterly
Psorergates simplex M, R Quarterly
Radfordia affinis M, R Quarterly
Rodentolepis spp. M, R Quarterly
Spironucleus muris M, R Quarterly
Syphacia spp. M, R Quarterly
Trichomonads M, R Quarterly

Germ Free (GF) health standard

    Core testing program Opportunist screening IVC PCR testing IVC exhaust air duct testing Isolator microbial monitoring 16S bacterial RNA screening
Agent Species tested Applicable Not applicable Not applicable Not applicable Applicable Applicable
Viruses
Kilham Rat Virus (KRV) R Quarterly
Minute Virus of Mice (MVM) M Quarterly
Mouse Encephalomyelitis Virus (GDVII)/ Rat Theilovirus (RTV) M, R Quarterly
Mouse Hepatitis Virus (MHV) M Quarterly
Murine Norovirus (MNV) M Quarterly
Mouse Parvovirus (MPV) M Quarterly
Mouse Rotavirus (EDIM) M Quarterly
Pneumonia Virus(PVM) M, R Quarterly
Rat Coronavirus (RCV)/Sialodacryoadenitis Virus (SDAV) R Quarterly
Rat Minute Virus (RMV) R Quarterly
Rat Parvovirus (RPV) R Quarterly
Toolan's H1 Virus (TH1) R Quarterly
Ectromelia Virus M Quarterly
Hantaan Virus M, R Quarterly
K Virus M Quarterly
Lymphocytic Choriomeningitis Virus (LCM) M, R Quarterly
Mouse Adenovirus I and II (FL and K87) M, R Quarterly
Mouse Cytomegalovirus (MCMV) M Quarterly
Polyoma Virus M Quarterly
Reovirus (Reo 3) M, R Quarterly
Sendai Virus M, R Quarterly
Thymic Virus M Semiannually
Lactate Dehydrogenase Elevating Virus (LDHV) M Annually
Bacteria, mycoplasma, fungi All bacterial, mycoplasma, fungal contaminants monitored weekly Bacterial RNA detection performed monthly
Beta hemolytic Streptococcus (non Group D) M, R Quarterly
Bordetella bronchiseptica M, R Quarterly
Cilia Associated Respiratory Bacillus (CARB) M, R Quarterly
Citrobacter rodentium M Quarterly
Clostridium piliforme M, R Quarterly
Corynebacterium bovis M, R Quarterly
Corynebacterium kutscheri M, R Quarterly
Helicobacter spp. M, R Quarterly
Klebsiella oxytoca M, R Quarterly
Klebsiella pneumoniae M, R Quarterly
Mycoplasma pulmonis M, R Quarterly
Pasteurella multocida M, R Quarterly
Pasteurella pneumotropica M, R Quarterly
Pneumocystis spp. M, R Quarterly
Proteus spp. M, R Quarterly
Pseudomonas aeruginosa M, R Quarterly
Salmonella spp. M, R Quarterly
Staphylococcus aureus M, R Quarterly
Streptococcus pneumoniae M, R Quarterly
Segmented Filamentous Bacteria (SFB) M, R Annually
Streptobacillus moniliformis M, R Annually
Parasites
Aspiculuris tetraptera M, R Quarterly
Eimeria spp. M, R Quarterly
Encephalitozoon cuniculi M, R Quarterly
Entamoeba spp. M, R Quarterly
Giardia muris M, R Quarterly
Hymenolepis spp. M, R Quarterly
Myobia musculi M, R Quarterly
Myocoptes musculinus M, R Quarterly
Ornithonyssus bacoti M, R Quarterly
Polyplax spinulosa M, R Quarterly
Psorergates simplex M, R Quarterly
Radfordia affinis M, R Quarterly
Rodentolepis spp. M, R Quarterly
Spironucleus muris M, R Quarterly
Syphacia spp. M, R Quarterly
Trichomonads M, R Quarterly