- Knockout of the uracil DNA glycosylase (Ung) gene
- This line is useful for the in vivo study of Ung function and DNA mismatch mutation during DNA synthesis.
- Mice develop B cell lymphoma and are defective in their immune response.
Origin: The Ung knockout mouse was developed in the laboratory of Thomas Lindahl at the London Research Institute. A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were interbred to generate homozygous Ung-/- mice. Founder lines were backcrossed to C57BL/6. Taconic received embryos from CRT in 2015.
Source: Cancer Research Technology (CRT)
Nilsen et al., 2003. Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas. Oncogene., 22:5381-86