- Contains a disruption of the recombination activating gene 2 (Rag2)
- Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
- Useful for vaccine development, transplantation studies and hematopoiesis research.
Genetic Background: C57BL/10
The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. NIAID received Rag2 mice from the Alt lab and backcrossed 15 generations to B10.D2 and then further backcrossed for five generations to C57BL/10.
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement
, Cell, 68: 855-867