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Pld3 - Model 13775 - KO

Constitutive Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 13775
    C57BL/6-Pld3tm1.2Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
  • Pld3 exon 4 contains the translation initiation codon.
  • Exons 7-9 have been flanked by loxP sites.
  • The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
  • The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
  • Deletion of exons 7-9 should result in loss of function of the Pld3 gene by deleting a part of Phospholipase D/Transphosphatidylase domain.
  • The remaining recombination sites are located in intron regions of the genome.
  • The corresponding conditional KO allele is available as line 13774.
  • Data mining and Design performed in 2004.

Gene Family:

Phosphatase

Genetic Background:

C57BL/6

Gene Symbol:

Pld3

Gene Accession Number:

NM_011116

Allele Type:

KO

Source:

MCI mol bio pac uploaded

Species:

Mouse

Official Gene Name:

phospholipase D family, member 3

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

For-Profit Pricing:

Price per Line ($)
$30,000

Non-Profit Pricing:

Price per Line ($)
$5,500

For-Profit Pricing:

Price per Line (€)
€27,300

Non-Profit Pricing:

Price per Line (€)
€5,005