The NFκB-RE-luc mouse was developed by Caliper Life Sciences. The model was created by microinjecting a transgene containing 6 NFκB-responsive elements (RE) from the CMVα (immediate early) promoter placed upstream of a basal SV40 promoter, and a modified firefly luciferase cDNA (Promega pGL3). This transgene was microinjected into BALB/cJ zygotes. The resultant mice from founder line 31 were bred to BALB/cJ mice. Taconic received stock from Caliper in 2010, and the line was embryo transfer derived. In the production colony, the line is maintained by mating mice which are wild type to those which are hemizygous for the luciferase transgene.
There is no specific publication describing the generation of these mice, but multiple publications exist demonstrating applications using this model and a similar model generated by Dr. Blomhoff. See reference list.
Figure 1. The mice (n=3) were imaged at T= 0 (pretreatment) and 4, 7, and 24 hours following intraperitoneal injection of LPS (1mg/kg) (A). Photons/sec quantified from liver and whole body. The data (B) represent mean fold of induction
Figure 2. The mice (n=3) were imaged at T=0 (pretreatment) and 4, 7, and 24 hours following intraperitoneal injection of TNFα (2 ìg/mouse) (A). Photons/sec quantified from liver and whole body. The data (B) represent mean fold of induction.
Figure 3. Basal expression. Ubiquitious expression in the whole body, strong signals in the abdominal and thoracic regions. The whole body signal intensity is 3x109 photons/sec.