This targeted mutation strain carries a replacement of the mouse Abcc2 gene which encodes for the multidrug resistance protein (MRP2) with the corresponding human orthologous gene.
Useful to study the role of human MRP2 in drug bioavailability.
Origin: The Humanized MRP2 Mouse was developed by Taconic in collaboration with CXR Biosciences. It was created through a targeted replacement of mouse Mrp2 Exon 2 with a cDNA construct containing human MRP2 Exons 2-32 in C57BL/6NTac-derived ES cells. This leads to the expression of a fusion protein of amino acids encoded by mouse Exons 1 and human Exons 2 to 32 driven by the mouse Mrp2 promoter, so that the potential mouse leader sequence encoded by Exon 1 is retained. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.
Scheer N, Balimane P, Hayward MD, Buechel S, Kauselmann G, Wolf CR. Generation and Characterization of a Novel MRP2 Humanized Mouse Line. Drug Metab Dispos. 2012 Aug 23
Wen X, Buckley B, McCandlish E, Goedken MJ, Syed S, Pelis R, Manautou JE, Aleksunes LM. Transgenic expression of the human MRP2 transporter reduces cisplatin accumulation and nephrotoxicity in Mrp2-null mice. Am J Pathol. 2014 184(5):1299-308