Mir449abc - Model 9983 - KO

• Targeting vector has been generated using C57BL/6J DNA and has been transfected into C57BL/6N Tac ES cell line.
• The cluster containing Mir449a, b and c has been flanked by loxP sites.
• The cluster Mir449 is embedded into intron 2 of the predicted mouse gene Cdc20b (ENSMUSG00000078926).
• Mir449c is located 206 bp downstream of the predicted exon2 of the Cdc20b gene. The proximal LoxP site has been inserted within these 206 bp.
• The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
• The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
• Datamining and Design performed in 2009.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.