The HRN™ mouse was developed in the laboratory of C. Roland Wolf of the Ninewells Hospital & Medical School. The model was created by targeting the Por gene to generate a floxed allele in GK129/1 embryonic stem cells derived from 129P2 mice and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for one generation. Mice heterozygous for the floxed Por allele were intercrossed to generate mice homozygous for the floxed Por allele on a mixed B6;129P2 background. The Alb-cre transgene was developed in the laboratory of Mark A. Magnuson at Vanderbilt University School of Medicine by microinjecting Cre recombinase gene under the control of the rat albumin enhancer/promoter into B6D2F2 zygotes. Mice homozygous for the floxed Por allele were bred to carriers for the Alb-cre transgene to generate HRN™ mice. The HRN™ model was backcrossed to C57BL/6J a total of 6 generations (N6). Taconic received stock from CXR Biosciences in April 2007. The mice were backcrossed to C57BL/6NTac (N7) and embryo transfer derived. The colony was maintained through mating of mice that are homozygous for the floxed Por allele and homozygous for the Alb-cre transgene.
Henderson CJ, Otto DME, Carrie D, Magnuson MA, McLaren AW, Rosewell I, Wolf CR. (2003) Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase. J Biol Chem 278(15):13480-13486.