Constitutive Knockout

EZcohort® Models
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This model is available for immediate cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 7353
    B6.Cg-Portm1Wolf Tg(Alb-cre)21Mgn N7
  • HRN™ stands for Hepatic cytochrome P450 Reductase Null
  • Mice are homozygous for both the floxed Por allele and the Alb-cre transgene
  • Exhibits liver-specific conditional disruption of the Por gene; POR is the electron donating enzyme for all cytochrome P450 enzymes
  • Lacks first pass metabolism by P450 enzymes in the liver
  • Can be used to determine the role of hepatic P450 metabolism in drug disposition
  • Useful for in vivo efficacy studies using smaller amounts of compound
  • Can provide information on whether parent or metabolite compounds are responsible for observed efficacy or toxic effects

Genetic Background:

C57BL/6 Background


The HRN™ mouse was developed in the laboratory of C. Roland Wolf of the Ninewells Hospital & Medical School. The model was created by targeting the Por gene to generate a floxed allele in GK129/1 embryonic stem cells derived from 129P2 mice and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for one generation. Mice heterozygous for the floxed Por allele were intercrossed to generate mice homozygous for the floxed Por allele on a mixed B6;129P2 background. The Alb-cre transgene was developed in the laboratory of Mark A. Magnuson at Vanderbilt University School of Medicine by microinjecting Cre recombinase gene under the control of the rat albumin enhancer/promoter into B6D2F2 zygotes. Mice homozygous for the floxed Por allele were bred to carriers for the Alb-cre transgene to generate HRN™ mice. The HRN™ model was backcrossed to C57BL/6J a total of 6 generations (N6). Taconic received stock from CXR Biosciences in April 2007. The mice were backcrossed to C57BL/6NTac (N7) and embryo transfer derived. The colony was maintained through mating of mice that are homozygous for the floxed Por allele and homozygous for the Alb-cre transgene.





Initial Publication:

Henderson CJ, Otto DME, Carrie D, Magnuson MA, McLaren AW, Rosewell I, Wolf CR. (2003) Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase. J Biol Chem 278(15):13480-13486.

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