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Hivep3 - Model 10494 - PM

Point Mutation

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10494
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Hivep3 exon 4 contains the translation initiation site.
  • K886A, K887A and K888A mutations have been introduced into exon 4 (aminoacid: KLPPKKKRLR to KLPPAAARLR; base: AAGAAAAAG to GCCGCTGCC).
  • The positive selection marker (Puromycin resistance) has been flanked by F3 sites and has been inserted within intron 4.
  • The constitutive PM allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The constitutive PM line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The remaining FRT recombination site will be located in a non-conserved region of the genome.
  • Datamining and Design performed in 2010.

Gene Family:

Transcription factor

Genetic Background:

C57BL/6

Gene Symbol:

Hivep3

Gene Accession Number:

ENSMUST00000106307

Allele Type:

PM

Source:

TaconicArtemis

Species:

Mouse

Official Gene Name:

human immunodeficiency virus type I enhancer binding protein 3

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.