C57BL/6 mice deficient in Kb were generated by homologous recombination in the laboratory of Dr. Francois Lemonnier of The Institut Pasteur, Paris France.
Generation of H-2 Kb Targeted Mutation mice-The Kb gene was disrupted by replacing the 2nd and 3rd exons with an HPRT minigene inserted in the opposite transcriptional orientation. The disrupted gene was introduced into an E14TGa(H-2b and b2ma) HPRT deficient embryonic stem cell of 129/OLA origin. After injection into B6 blastocysts, the resultant germline chimeras were backcrossed twice to C57BL/6Ji mice and offspring heterozygous for the H2-Kb knockout allele were crossed to generate H2-Kb deficient mice (Kb -/-). Inactivation of the H2-Kbgene was confirmed by Southern blot analysis on tail DNA and by FACS analysis. The targeted mutation mice were subsequently backcrossed to C56BL/6Ji mice another 10 times before being intercrossed to achieve homozygosity . Six pairs of the N12 homozygous mice were transferred from Dr. Lemonnier to the NIAID repository at Taconic in 2001 where they were embryo transfer derived.
Pascolo S, Bervas N, Ure JM, Smith AG, Lemmonier FA and Pérarnau B. HLA-A2.1-restricted education and cytolytic activity of CD8+ T lymphocytes from β2-microglobulin(β2m) HLA-2.1 monochain trangenic H2Dbβ2m double knockout mice. J. Exp. Med. 1997. 185: 2043-2051
Pérarnau B, Saron MF, San Martin BR, Bervas N, Ong H, Soloski MJ, Smith AG, Ure JM, Gairin JE and Lemonnier FA. Single H-2Kb, H-2Db and double H-2KbDb knockout mice: peripheral CD8+ T cell repertoire and antilymphocytic choriomeningitis virus cytolytic responses. Eur. J. Immunol. 1999. 29: 1243-1252