H2-K^b Knockout

Origin:

C57BL/6 mice deficient in K^b were generated by homologous recombination in the laboratory of Dr. Francois Lemonnier of The Institut Pasteur, Paris France.

Generation of H-2 K^b Targeted Mutation mice-The K^b gene was disrupted by replacing the 2nd and 3rd exons with an HPRT minigene inserted in the opposite transcriptional orientation. The disrupted gene was introduced into an E14TGa(H-2b and b2ma) HPRT deficient embryonic stem cell of 129/OLA origin. After injection into B6 blastocysts, the resultant germline chimeras were backcrossed twice to C57BL/6Ji mice and offspring heterozygous for the H2-K^b knockout allele were crossed to generate H2-K^b deficient mice (K^b -/-). Inactivation of the H2-K^bgene was confirmed by Southern blot analysis on tail DNA and by FACS analysis. The targeted mutation mice were subsequently backcrossed to C56BL/6Ji mice another 10 times before being intercrossed to achieve homozygosity . Six pairs of the N12 homozygous mice were transferred from Dr. Lemonnier to the NIAID repository at Taconic in 2001 where they were embryo transfer derived.

Availability:

Color:

Species:

Initial Publication:

Pascolo S, Bervas N, Ure JM, Smith AG, Lemmonier FA and Pérarnau B. HLA-A2.1-restricted education and cytolytic activity of CD8+ T lymphocytes from β2-microglobulin(β2m) HLA-2.1 monochain trangenic H2D^bβ2m double knockout mice. J. Exp. Med. 1997. 185: 2043-2051

Pérarnau B, Saron MF, San Martin BR, Bervas N, Ong H, Soloski MJ, Smith AG, Ure JM, Gairin JE and Lemonnier FA. Single H-2K^b, H-2D^b and double H-2K^bD^b knockout mice: peripheral CD8+ T cell repertoire and antilymphocytic choriomeningitis virus cytolytic responses. Eur. J. Immunol. 1999. 29: 1243-1252