The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
Galr3 exon 1 contains the translation initiation codon.
Exon 1 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination.
Deletion of exon 1 should result in loss of function of the Galr3 gene by deleting the translation initiation codon.
The remaining recombination sites are located in intron regions of the genome.
The corresponding constitutive KO allele is available as line 7267.
