Cryopreserved Model

  • Model #
  • Genotype
  • Nomenclature
  • 13802
  • The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
  • Galr3 exon 1 contains the translation initiation codon.
  • Exon 1 has been flanked by loxP sites.
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination.
  • Deletion of exon 1 should result in loss of function of the Galr3 gene by deleting the translation initiation codon.
  • The remaining recombination sites are located in intron regions of the genome.
  • The corresponding constitutive KO allele is available as line 7267.
  • Datamining and Design performed in 2004.

Species:

Mouse