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The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
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The H101R point mutation was introduced into Gabra1 exon 4.
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The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 3.
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Chimeric mice were produced and germ line transmission achieved.
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The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
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The H101R point mutation in exon 4 abolishes benzodiazepine binding of the Gabra1 H101R receptor, but keeps the Gabra1 function otherwise normal.
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Gabra1 H101R constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
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See R.M. McKernan et al, Nat Neurosci. 2000 Jun;3(6):587-92, for further details.
Gene Family:
Receptor Genetic Background:
B6:129S6 Gene Symbol:
Gabra1 Gene Accession Number:
NM_010250 Allele Type:
PM Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1
