Gabra1 - Model 7088 - PM

Point Mutation Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

B6;129S6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 7088
    C57BL/6-Gabra1tm1.1(H101R)Rmck>
  • The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
  • The H101R point mutation was introduced into Gabra1 exon 4.
  • The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 3.
  • Chimeric mice were produced and germ line transmission achieved.
  • The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
  • The H101R point mutation in exon 4 abolishes benzodiazepine binding of the Gabra1 H101R receptor, but keeps the Gabra1 function otherwise normal.
  • Gabra1 H101R constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
  • See R.M. McKernan et al, Nat Neurosci. 2000 Jun;3(6):587-92, for further details.

Gene Family:

Receptor

Genetic Background:

B6:129S6

Gene Symbol:

Gabra1

Gene Accession Number:

NM_010250

Allele Type:

PM

Source:

Merck Research Laboratories

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1

For-profit users: Commercial users may purchase available GEM Collection models by signing the GEM Collection Commercial Terms of Sale and providing a PO. The GEM Collection Commercial Terms of Sale grant perpetual use rights.
Non-profit users: Non-profit users must provide a scientific workplan and execute an MTA prior to accessing a GEM Collection Model.

Non-profit order process is as follows:

  1. Non-profit Investigator visits the Taconic website and identifies GEM of interest.
  2. Non-profit Investigator inquires on Taconic website.
  3. Taconic will answer questions about the GEM lines and the process.
  4. Taconic sends request for MTA.
  5. Non-profit Investigator provides scientific workplan answering 4 basic questions.
  6. MTA is issued for non-profit Investigator/institution review and signature.
  7. MTA is executed by all parties and release of model is approved.
  8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery and shipment costs.

Taconic begins cryorecovery upon receipt of PO and delivers model to requestor. It is the requestor's obligation to determine that the GEM meets their research needs.

Access to this model for non-profit customers requires approval of a third-party Material Transfer Agreement (MTA). The terms of this MTA are non-negotiable; an example agreement is available so that you may preview the terms. MTA approval may require 4-6 months due to circumstances outside our control. Once approved and the purchase confirmed, order fulfillment will require an additional 14-16 weeks. If you wish to continue your interest in this model, please complete the GEM Collection Inquiry Form. In addition, Taconic also offers complete model generation services that may allow for a reduced timeline to delivery of a comparable model. If you are interested in learning more, please request a GEMs Design Consultation.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

For-Profit Pricing:

Price per Line ($)
$30,000

Non-Profit Pricing:

Price per Line ($)
$5,500

For-Profit Pricing:

Price per Line (€)
€27,300

Non-Profit Pricing:

Price per Line (€)
€5,005