Ffar4 - Model 14386- cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 14386
    C57BL/6-Ffar4tm1.1Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 1 contains the translation initiation codon.
  • Exon 1 and approx. 1.5 kb of sequence upstream of exon 1 (promoter region) have been flanked by loxP sites (size of loxP-flanked region: 3.2 kb).
  • The positive selection marker (Puromycin resistance - PuroR) has been flanked by FRT sites and inserted into intron 1.
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination.
  • Deletion of exon 1 and proximal promoter should result in loss of function of the Ffar4 gene, by preventing transcription of the Ffar4 mRNA and by deleting the transmembrane domains 1 to 4.
  • The remaining recombination sites will be located in non-conserved regions of the genome.
  • Datamining and Design performed 2014.

Gene Family:

Receptor

Genetic Background:

C57BL/6

Gene Symbol:

Ffar4

Gene Accession Number:

ENSMUST00000067098

Allele Type:

cKO

Source:

TaconicArtemis

Species:

Mouse

Official Gene Name:

free fatty acid receptor 4

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
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