The Dr3 knockout mouse was developed in the laboratory of Eddie Wang at the Imperial Cancer Research Fund, Lincoln's Inn Fields, London. A DR3 targeting vector, replacing the entire DR3 coding region with a loxP flanked resistance cassette, was transfected into GK129 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to yield mice heterozygous for the mutant allele. Heterozygous mice were interbred to generate homozygous Dr3-/- mice. Taconic received embryos from CRT in 2015.
Wang et al. 2001. DR3 regulates negative selection during thymocyte development. Molecular and Cellular Biology. 21:3451-61.