- Knockout of the tumor necrosis factor receptor superfamily, member 25 Tnfrsf25 (also known as Dr3) gene
- Dr3 mediates regulation of the cell division cycle.
- Negative selection and anti-CD3-induced apoptosis are significantly impaired in Dr3-null mice.
Genetic Background: 129;C57BL/6
Origin: The Dr3 knockout mouse was developed in the laboratory of Eddie Wang at the Imperial Cancer Research Fund, Lincoln's Inn Fields, London. A DR3 targeting vector, replacing the entire DR3 coding region with a loxP flanked resistance cassette, was transfected into GK129 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to yield mice heterozygous for the mutant allele. Heterozygous mice were interbred to generate homozygous Dr3-/- mice. Taconic received embryos from CRT in 2015.
Source: Cancer Research Technology (CRT)