This model carries a deletion of fourteen full-length mouse Cyp2c genes
Contains a cre-mediated deletion of the following genes in the Cyp2c cluster: Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50, 2c70.
Mouse Cyp2c44, which is physically located outside of the Cyp2c Cluster has not been deleted.
In humans, CYP2C enzymes metabolize up to 30% of marketed drugs
Useful in dissecting the contribution of the Cyp2c family to total bioavailability of a drug
Genetic Background: C57BL/6N Background
Origin: The Cyp2c Knockout Mouse was developed by Taconic in collaboration with CXR Biosciences. The model was created by first replacing mouse exon 1 of Cyp2c55 with a neomycin cassette containing a loxP site and subsequently inserting a hygromycin cassette with a loxP site into intron 6 of the Cyp2c70 gene through gene targeting in C57BL/6NTac-derived ES cells. Transfection of the ES cells with cre recombinase resulted in the deletion of Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50 and 2c70. The targeted ES cells were injected into BALB/cJBomTac blastocysts. Resultant chimeras were backcrossed to C57BL/6NTac wild type mice. The colony was maintained by mating homozygotes.
Scheer N, Kapelyukh Y, Chatham L, Rode A, Buechel S, Wolf CR. Generation and Characterization of Novel Cytochrome P450 Cyp2c Gene Cluster Knockout and CYP2C9 Humanized Mouse Lines. Mol Pharmacol. 2012 Aug 23