The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6N Tac ES cell line.
Creg1 exon 1 contains the translation initiation codon.
Exon 1has been flanked by loxP sites including the FRT site flanked neomycin resistance gene cassette.
The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
The conditional KO line has been derived using C57BL/6NTac animals.
The constitutive KO allele can be generated by Cre-mediated recombination.
Deletion of exon 1 should result in loss of function of the Creg1 gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2009.
Gene Family: Repressor
Genetic Background: C57BL/6
Gene Symbol: Creg1
Gene Accession Number: NM_011804
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: cellular repressor of E1A-stimulated genes 1