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This targeted mutation strain carries a deletion of the mouse aryl hydrocarbon receptor (Ahr) gene.
Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity and efficacy in humans.
Mice which are homozygous knockouts for the Ahr gene are smaller than heterozygotes or wild types. As described in the literature, dead newborn and missing litters are common in Ahr knockout animals, with some incidence of death after weaning also seen. Taconic recommends that this line be ordered no earlier than 6 weeks of age, and 8-10 weeks of age or older may be preferable, particularly if transit times will be longer than 2 days.
- The Ahr Knockout mouse has immune system abnormalities. Taconic recommends maintaining this model under more restrictive husbandry conditions to minimize clinical problems.
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
The Ahr Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing an AHR humanized mouse line (Model 9165) with a Cre deleter mouse. The Humanized AHR Mouse model was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The Ahr Knockout Mouse model was derived from the Humanized AHR Mouse line through a Cre-mediated deletion of the human AHR sequence. Mouse exon 3 is deleted in the knockout, resulting in an out of frame splicing of exons 2 to exon 4. The absence of functional Ahr protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.
Not published yet.