Ahr Knockout Mouse

Constitutive Knock Out

Ahr Constitutive Knock Out Mouse Model
MPF health status to be discontinued effective January 1, 2018. EF health status will be available.

  • Model #
  • Genotype
  • Nomenclature
  • 9166-F
    ko/ko
    C57BL/6-Ahrtm1.2Arte
  • 9166-M
    ko/ko
    C57BL/6-Ahrtm1.2Arte
  • This targeted mutation strain carries a deletion of the mouse aryl hydrocarbon recpetor (Ahr) gene.
  • Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity and efficacy in humans.
  • Mice which are homozygous knockouts for the Ahr gene are smaller than heterozygotes or wild types. As described in the literature, dead newborn and missing litters are common in Ahr knockout animals, with some incidence of death after weaning also seen. Taconic recommends that this line be ordered no earlier than 6 weeks of age, and 8-10 weeks of age or older may be preferable, particularly if transit times will be longer than 2 days.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin:

The Ahr Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing an AHR humanized mouse line (Model 9165) with a Cre deleter mouse. The Humanized AHR Mouse model was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The Ahr Knockout Mouse model was derived from the Humanized AHR Mouse line through a Cre-mediated deletion of the human AHR sequence. Mouse exon 3 is deleted in the knockout, resulting in an out of frame splicing of exons 2 to exon 4. The absence of functional Ahr protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.

Color:

Black

Species:

Mouse

Initial Publication:

Not published yet.


Conditions of Use for Emerging Models
Emerging Models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless a breeding or cross-breeding agreement has been legally executed. Please contact Taconic Customer Service for details.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
Conditions of Use - Printable Version