Humanized AHR Mouse

Targeted Replacement

Humanized AHR Targeted Replacement Mouse Model
EZcohort™ Models
The easiest way to acquire and breed cryopreserved GEMs

  • Model #
  • Genotype
  • Nomenclature
  • 9165-F
  • 9165-M
  • This targeted mutation strain carries a replacement of the mouse aryl hydrocarbon receptor (Ahr) gene with the corresponding human orthologous gene.
  • Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy in humans.


The Humanized AHR Mouse was developed by Taconic in collaboration with CXR Biosciences. It was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. This leads to the expression of a fusion protein of amino acids encoded by mouse Exons 1 and 2 and human Exons 3 to 11 driven by the mouse Ahr promoter. The amino acids encoded by Exon 1 and 2 of mouse and human AHR are highly conserved, with only two changes in Exon 1. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.





Initial Publication:

Not published yet.

Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.