C57BL/6, 129P2 Mixed Background
Origin: The COX-1 mouse was developed in the laboratories of Robert Langenbach at NIEHS and Oliver Smithies at University of North Carolina in 1995. The model was created by targeting the Ptgs1 gene in E14TG2a embryonic stem cells derived from 129P2/OlaHsd mice and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to C57BL/6J mice. Taconic received stock in September 1998 for embryo transfer derivation into the NIEHS repository. The model was transferred from NIEHS to Taconic in 2002 and derived by embryo transfer. The line is maintained on a mixed B6;129P2 background by breeding homozygous males to heterozygous females. A separate wild type colony is maintained.
Genetics: Wildtype for Nnt mutation
Initial Publication: Langenbach R, Morham SG, Tiano HF, Loftin CD, Ghanayem BI, Chulada PC, Mahler JF, Lee CA, Goulding EH, Kluckman KD, Kim HS, Smithies O. (1995) Prostaglandin Synthase 1 Gene Disruption in Mice Reduces Arachidonic Acid-Induced Inflammation and Indomethacin-Induced Gastric Ulceration. Cell, 83(3):483-492.
Label License: Conditions of Use for Taconic Transgenic Models™
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