Constitutive Knock Out/Random Transgenic

Rag2/OT-II Constitutive Knock Out/Random Transgenic Mouse Model

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 1896-F
    B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
  • 1896-M
    B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
  • Carries a transgene that encodes for a T cell receptor (Valpha2 and Vbeta5) specific for a chicken ovalbumin peptide (323-339) presented by the MHC class II molecule H2-Ab1 (I-Ab)
  • Deficient in the recombination activating gene 2 (Rag2) and therefore does not develop mature T and B cells expressing endogenous T cell receptors
  • Virtually all CD4+ cells express the transgene
  • Useful as a source of homogenous donor CD4+ cells for in vivo adoptive transfer studies to investigate T cell development, memory, activation and tolerance

Genetic Background:

C57BL/6 Background


The OT-II microinjected model was developed by Dr. Francis Carbone of Monash Medical School and Dr. William R. Heath of the Walter and Eliza Hall Institute. The model was created by microinjecting the rearranged Valpha2 and Vbeta5 T cell receptor into B6 x B6.C-H2bm1 blastocysts. Taconic received stock from Keio University in June 2001, and the mice were rederived by backcrossing to C57BL/6NTac. OT-II transgenic mice were crossed with a homozygous C57BL/6NTac Rag2 targeted mutation model (RAGN12-M), and then intercrossed for homozygosity at the Rag2 allele. The model was rederived by embryo transfer and is maintained by mating of mice that are homozygous for Rag2 disruption and heterozygous for the OT-II transgene to mice that are homozygous for the Rag2 disruption and wild type for OT-II.


Due to poor production by ko/ko;tg/tg x ko/ko;tg/tg setups, Taconic will transition the colony to the following mating format: ko/ko;tg/wt (male or female) by ko/ko;wt/wt. During the transition period, inventory will include mice that are homozygous and hemizygous for the OT-II transgene. Eventually, only mice that are hemizygous for the OT-II transgene will be available. A review of flow cytometry analysis for hemizygous and homozygous OT-II mice showed that T cell numbers and TCR expression levels were similar for the two genotypes. We expect minimal to no functional difference between the genotypes in typical experiments.





Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.

Barnden MJ, Allison J, Heath WR, Carbone FR. (1998) Defective TCR expression in transgenic mice constructed using cDNA-based α- and Β-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol 76:34-40.

Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.